PXD036178

PXD036178 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of stool samples from preterm neonates with mass spectrometry for predicting necrotizing enterocolitis
Description	This study aimed to investigate the potential of fecal proteomics signatures for NEC prediction. Stools were collected from NEC infants up to 10 days before their diagnosis and matched with those from non-NEC controls for three tested periods. Stool samples were analyzed with liquid chromatography-tandem mass spectrometry using SWATH acquisition and a cross-compatible proteomic software platform to perform label-free quantification. ROC curves and principal component analysis were used to explore discriminating information provided by potential markers and sets of markers were evaluated with classical clustering and classification methods to evaluate their capacity to sort the samples giving to their actual outcomes. We identified a specific series of markers that predicted all NEC from non-NEC infants at least a week in advance. Our study shows that multiplexed proteomic signature detection constitutes a favorable approach for the early detection of NEC.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:38:50.839.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Gagne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-21 03:38:46	ID requested
1	2023-03-11 00:01:26	announced
⏵ 2	2023-11-14 08:38:51	announced	2023-11-14: Updated project metadata.

Gagn, é D, Shajari E, Thibault MP, No, ë, l JF, Boisvert FM, Babakissa C, Levy E, Gagnon H, Brunet MA, Grynspan D, Ferretti E, Bertelle V, Beaulieu JF, Proteomics Profiling of Stool Samples from Preterm Neonates with SWATH/DIA Mass Spectrometry for Predicting Necrotizing Enterocolitis. Int J Mol Sci, 23(19):(2022) [pubmed]

submitter keyword: SWATH, mass spectrometry, necrotizing enterocolitis, intestines, preterm infants, stool, DDA, detection, DIA, proteomic signature,Proteomics, diagnosis, biomarkers, neonates

Jean-François Beaulieu
contact affiliation	Département d'immunologie et de biologie cellulaire, Faculté de médecine et des sciences de la santé, Université de Sherbrooke
contact email	jean-francois.beaulieu@usherbrooke.ca
lab head
David Gagne
contact affiliation	Departement d'immunologie et de biologie cellulaire, Faculté de médecine et des sciences de la santé, Université de Sherbrooke
contact email	david.gagne@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036178

PRIDE project URI

• PRIDE
  1. PXD036178
     1. Label: PRIDE project
     2. Name: Proteomics analysis of stool samples from preterm neonates with mass spectrometry for predicting necrotizing enterocolitis