PXD036168

PXD036168 is an original dataset announced via ProteomeXchange.

Title	Presence of ceramidase activity in electronegative LDL
Description	Electronegative LDL (LDL(-)) is a minor modified fraction of human plasma LDL with several atherogenic properties. Among them, LDL(-) has increased content of bioactive lipid mediators, such as lysophosphatidylcholine (LPC), non-esterified fatty acids (NEFA), ceramide (Cer), and sphingosine (Sph), which are related to the presence in LDL(-) of some phospholipolytic activities, including platelet-activating factor acetylhydrolase (PAF-AH), phospholipase C (PLC) and sphingomyelinase (SMase). However, the activity of these enzymes does not explain the increased content of Sph, which should derive from Cer degradation. In the present study we analyzed the putative presence of a ceramidase (CDase) activity that could explain the increased content of Sph. Thin layer chromatography (TLC) and lipidomic analysis showed that Cer, Sph and NEFA spon-taneously increased in LDL(-) incubated alone at 37ºC, in contrast with native LDL(+). An inhibitor of neutral CDase prevented the formation of Sph and, in contrast, increased the content of Cer in LDL(-). In addition, a fluorescently-labelled Cer (NBD-Cer) was efficiently degraded by LDL(-) to form Sph and NEFA. These observations point to the existence of a CDase activity associated to LDL(-). However, neither proteomic analysis nor western blot detected the presence of a known CDase enzyme. Further studies are warranted to define the origin of the CDase activity detected in LDL(-).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:53:35.655.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesc Canals
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-19 01:29:48	ID requested
1	2023-01-09 07:37:34	announced
⏵ 2	2023-11-14 07:53:36	announced	2023-11-14: Updated project metadata.

Puig N, Rives J, Estruch M, Aguilera-Simon A, Rotllan N, Camacho M, Colom, é N, Canals F, S, á, nchez-Quesada JL, Benitez S, Presence of Ceramidase Activity in Electronegative LDL. Int J Mol Sci, 24(1):(2022) [pubmed]

submitter keyword: electronegative LDL

ceramide

sphingosine

ceramidase

sphingomyelinase

José Luis Sánchez-Quesada
contact affiliation	Cardiovascular Biochemistry Group, Research Institute of the Hospital de la Santa Creu i Sant Pau (IIB Sant Pau), Barcelona, Spain
contact email	jsanchezq@santpau.cat
lab head
Francesc Canals
contact affiliation	Vall Hebron Institute of Oncology
contact email	fcanals@vhio.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD036168

PRIDE project URI

• PRIDE
  1. PXD036168
     1. Label: PRIDE project
     2. Name: Presence of ceramidase activity in electronegative LDL