PXD036164

PXD036164 is an original dataset announced via ProteomeXchange.

Title	proteomic analysis of the formalin-fixed paraffin embedded (FFPE) renal tissue between steroid-sensitive and steroid-resistant (SR) children FSGS patients
Description	In children, focal segmental glomerulosclerosis (FSGS) is the main cause of steroid resistant nephroticsyndrome (SRNS). To identify specific candidates and the mechanism of steroid resistance, we examined the formalin-fixed paraffin embedded (FFPE) renal tissue protein profiles via liquid chromatography tandem mass spectrometry (LC-MS/MS). Renal biopsies from seven steroid-sensitive (SS) and eleven steroid-resistant (SR) children FSGS patients were obtained. We examined the formalin-fixed paraffin embedded (FFPE) renal tissue protein profiles via liquid chromatography tandem mass spectrometry (LC-MS/MS). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and Gene Ontology (GO) analysis, as well as the construction of protein-protein interaction (PPI) network were performed. Two proteins were further valiadated by immunohistochemistry staining in FSGS patients and mice models. In total, we quantified 4794 proteins, of which 325 were found to be differentially expressed proteins (DEPs) between the SS and SR group (foldchange ≥ 2, P＜0.05). The results of GO revealed that the most significant up-regulated proteins were primarily related to protein transportation, regulation of the complement activation process and cytolysis. Moreover, clustering analysis showed differences in the pathways (lysosome, terminal pathway of complement) between the two groups. Among these potential candidates, validation analyses for LAMP1 and ACSL4 were conducted. LAMP1 was observed to have a higher expression in glomerulus, while ACSL4 was expressed more in tubular epithelial cells.
HostingRepository	PRIDE
AnnounceDate	2022-11-19
AnnouncementXML	Submission_2022-11-18_21:08:43.994.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JiajiaNi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-19 00:40:28	ID requested
⏵ 1	2022-11-18 21:08:44	announced

Dataset with its publication pending

submitter keyword: kidney tissue,human, LC-MS/MS

HongXu
contact affiliation	Department of Nephrology, Children's Hospital of Fudan University, National Pediatric Medical Center of China, Shanghai, China.
contact email	hxu@shmu.edu.cn
lab head
JiajiaNi
contact affiliation	Children's Hospital of Fudan University, National Pediatric Medical Center of China, Shanghai, China.
contact email	nijiajia089712@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036164
     1. Label: PRIDE project
     2. Name: proteomic analysis of the formalin-fixed paraffin embedded (FFPE) renal tissue between steroid-sensitive and steroid-resistant (SR) children FSGS patients