PXD036149 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Structure of Saccharomyces cerevisiae Mediator-RNA polymerase II pre-initiation complex for divergent transcription |
Description | This study has determined structure of transcription initiation complexes including a DNA-bound activator, RNA polymerase II (Pol II), and Mediator on a divergent promoter GAL80/SUT719 using a combination of cryo-EM and XL-MS analyses. Our cryo-EM single-particle analysis reveals a dimeric form of Med-PIC through the Mediator Tail module induced by the activator protein. Density of the upstream DNA bound to the Gal4-VP16 was identifiable along the Mediator Tail module, while XL-MS localized flexible regions that were not visible by cryo-EM analysis, such as activator-binding domains (ABDs and KIX). |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-22 |
AnnouncementXML | Submission_2024-05-22_01:15:53.752.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hee Jong Kim |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-18 07:56:57 | ID requested | |
⏵ 1 | 2024-05-22 01:15:54 | announced | |
Publication List
10.1016/j.molcel.2023.01.011; |
Gorbea Col, รณ, n JJ, Palao L, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K, Structural basis of a transcription pre-initiation complex on a divergent promoter. Mol Cell, 83(4):574-588.e11(2023) [pubmed] |
Keyword List
submitter keyword: enhancer, divergent transcription,transcription, Mediator, UAS, initiation, activator, RNA polymerase II |
Contact List
Kenji Murakami |
contact affiliation | University of Pennsyvania |
contact email | kenjim@pennmedicine.upenn.edu |
lab head | |
Hee Jong Kim |
contact affiliation | University of Pennsylvania |
contact email | heejong@heejong.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD036149 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036149
- Label: PRIDE project
- Name: Structure of Saccharomyces cerevisiae Mediator-RNA polymerase II pre-initiation complex for divergent transcription