PXD036145

PXD036145 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Changes in the spore proteome of Bacillus cereus in response to introduction of plasmids.
Description	Abstract: Fluorescent fusion proteins were expressed in Bacillus cereus to visualize the germino-some by introducing a plasmid that carries fluorescent fusion proteins of germinant receptor GerR subunits or germinosome scaffold protein GerD. The effects of plasmid insertion and re-combinant protein expression on the spore proteome were investigated. Proteomic analysis showed that overexpression of the target proteins had negligible effects on the spore proteome. However, plasmid-bearing spores displayed dramatic abundance changes of spore proteins in-volved in signaling and metabolism. Our findings indicate that introduction of a plasmid alone alters spore protein composition dramatically, with 993 proteins significantly down-regulated and 415 proteins significantly up-regulated among 3323 identified proteins. This shows that empty vector controls are more appropriate to compare proteome changes due to plasmid en-coded genes than is the wild-type strain, when using plasmid based genetic tools. Therefore, re-searchers should keep in mind that molecular cloning techniques can alter more than their in-tended targets in a biological system, and interpret results with this in mind.
HostingRepository	MassIVE
AnnounceDate	2022-10-14
AnnouncementXML	Submission_2022-10-14_02:28:51.578.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gertjan Kramer
SpeciesList	scientific name: Bacillus cereus; NCBI TaxID: 1396;
ModificationList	iodoacetamide - site C; L-methionine sulfoxide
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-18 06:30:18	ID requested
⏵ 1	2022-10-14 02:28:51	announced

Publication List

Xiaowei Gao, Bhagyashree N. Swarge, Winfried Roseboom, Yan Wang, Henk L. Dekker, Peter Setlow, Stanley Brul and Gertjan Kramer. settings Open AccessArticle Changes in the Spore Proteome of Bacillus cereus in Response to Introduction of Plasmids. Microorganisms 2022, 10(9), 1695.

Xiaowei Gao, Bhagyashree N. Swarge, Winfried Roseboom, Yan Wang, Henk L. Dekker, Peter Setlow, Stanley Brul and Gertjan Kramer. settings Open AccessArticle Changes in the Spore Proteome of Bacillus cereus in Response to Introduction of Plasmids. Microorganisms 2022, 10(9), 1695.

Keyword List

submitter keyword: Proteomics, Bacillus cereus, Spores, Molecular Biology, Plasmid, Overexpression, Germinosome

submitter keyword: Proteomics, Bacillus cereus, Spores, Molecular Biology, Plasmid, Overexpression, Germinosome

Contact List

Gertjan Kramer
contact affiliation	University of Amsterdam
contact email	g.kramer@uva.nl
lab head
Gertjan Kramer
contact affiliation	Universiteit van Amsterdam
contact email	gertjan.kramer@uva.nl
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090153/