PXD036133

PXD036133 is an original dataset announced via ProteomeXchange.

Title	Profiling the heme-binding proteomes of bacteria using chemical proteomics
Description	Heme is a cofactor with myriad roles and is essential to almost all living organisms. Accordingly, bacte-rial pathogens have developed numerous mechanisms to acquire heme from their hosts. Beyond classi-cal gas transport and catalytic functions, heme is increasingly appreciated as a tightly controlled signal-ling molecule regulating protein expression. However, heme acquisition, biosynthesis and regulation is poorly understood beyond a few model organisms, and the heme-binding proteome has yet to be fully characterised in bacteria. Yet as heme homeostasis is critical for bacterial survival, heme-binding pro-teins are promising drug targets. Herein we report a chemical proteomics method for global profiling of heme-binding proteins in live cells for the first time. Employing a panel of heme-based clickable and photoaffinity probes enabled the profiling of 32-54% of the known heme-binding proteomes in Gram-positive and Gram-negative bacteria, and revealed previously unknown potential heme interactors. This simple-to-implement profiling strategy could be interchangeably applied to different cell types and sys-tems and fuel future research into heme biology.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:16:54.324.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Isabel Wilkinson
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-17 00:56:44	ID requested
1	2023-12-06 16:11:42	announced
⏵ 2	2024-10-22 06:16:55	announced	2024-10-22: Updated project metadata.

Wilkinson IVL, Bottlinger M, El Harraoui Y, Sieber SA, Profiling the Heme-Binding Proteomes of Bacteria Using Chemical Proteomics. Angew Chem Int Ed Engl, 62(9):e202212111(2023) [pubmed]

10.1002/anie.202212111;

submitter keyword: cofactor,Bacterial pathogens, heme, LC-MS/MS

Stephan A. Sieber
contact affiliation	Centre for Functional Protein Assemblies, Technical University of Munich, Ernst-Otto-Fischer-Straße 8, D-85748 Garching, Germany.
contact email	stephan.sieber@tum.de
lab head
Isabel Wilkinson
contact affiliation	Technical University of Munich
contact email	isabel.wilkinson@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036133
     1. Label: PRIDE project
     2. Name: Profiling the heme-binding proteomes of bacteria using chemical proteomics