PXD036133 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Profiling the heme-binding proteomes of bacteria using chemical proteomics |
Description | Heme is a cofactor with myriad roles and is essential to almost all living organisms. Accordingly, bacte-rial pathogens have developed numerous mechanisms to acquire heme from their hosts. Beyond classi-cal gas transport and catalytic functions, heme is increasingly appreciated as a tightly controlled signal-ling molecule regulating protein expression. However, heme acquisition, biosynthesis and regulation is poorly understood beyond a few model organisms, and the heme-binding proteome has yet to be fully characterised in bacteria. Yet as heme homeostasis is critical for bacterial survival, heme-binding pro-teins are promising drug targets. Herein we report a chemical proteomics method for global profiling of heme-binding proteins in live cells for the first time. Employing a panel of heme-based clickable and photoaffinity probes enabled the profiling of 32-54% of the known heme-binding proteomes in Gram-positive and Gram-negative bacteria, and revealed previously unknown potential heme interactors. This simple-to-implement profiling strategy could be interchangeably applied to different cell types and sys-tems and fuel future research into heme biology. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:16:54.324.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Isabel Wilkinson |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-17 00:56:44 | ID requested | |
1 | 2023-12-06 16:11:42 | announced | |
⏵ 2 | 2024-10-22 06:16:55 | announced | 2024-10-22: Updated project metadata. |
Publication List
Wilkinson IVL, Bottlinger M, El Harraoui Y, Sieber SA, Profiling the Heme-Binding Proteomes of Bacteria Using Chemical Proteomics. Angew Chem Int Ed Engl, 62(9):e202212111(2023) [pubmed] |
10.1002/anie.202212111; |
Keyword List
submitter keyword: cofactor,Bacterial pathogens, heme, LC-MS/MS |
Contact List
Stephan A. Sieber |
contact affiliation | Centre for Functional Protein Assemblies, Technical University of Munich, Ernst-Otto-Fischer-Straße 8, D-85748 Garching, Germany. |
contact email | stephan.sieber@tum.de |
lab head | |
Isabel Wilkinson |
contact affiliation | Technical University of Munich |
contact email | isabel.wilkinson@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036133
- Label: PRIDE project
- Name: Profiling the heme-binding proteomes of bacteria using chemical proteomics