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PXD036133

PXD036133 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProfiling the heme-binding proteomes of bacteria using chemical proteomics
DescriptionHeme is a cofactor with myriad roles and is essential to almost all living organisms. Accordingly, bacte-rial pathogens have developed numerous mechanisms to acquire heme from their hosts. Beyond classi-cal gas transport and catalytic functions, heme is increasingly appreciated as a tightly controlled signal-ling molecule regulating protein expression. However, heme acquisition, biosynthesis and regulation is poorly understood beyond a few model organisms, and the heme-binding proteome has yet to be fully characterised in bacteria. Yet as heme homeostasis is critical for bacterial survival, heme-binding pro-teins are promising drug targets. Herein we report a chemical proteomics method for global profiling of heme-binding proteins in live cells for the first time. Employing a panel of heme-based clickable and photoaffinity probes enabled the profiling of 32-54% of the known heme-binding proteomes in Gram-positive and Gram-negative bacteria, and revealed previously unknown potential heme interactors. This simple-to-implement profiling strategy could be interchangeably applied to different cell types and sys-tems and fuel future research into heme biology.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:16:54.324.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIsabel Wilkinson
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-17 00:56:44ID requested
12023-12-06 16:11:42announced
22024-10-22 06:16:55announced2024-10-22: Updated project metadata.
Publication List
Wilkinson IVL, Bottlinger M, El Harraoui Y, Sieber SA, Profiling the Heme-Binding Proteomes of Bacteria Using Chemical Proteomics. Angew Chem Int Ed Engl, 62(9):e202212111(2023) [pubmed]
10.1002/anie.202212111;
Keyword List
submitter keyword: cofactor,Bacterial pathogens, heme, LC-MS/MS
Contact List
Stephan A. Sieber
contact affiliationCentre for Functional Protein Assemblies, Technical University of Munich, Ernst-Otto-Fischer-Straße 8, D-85748 Garching, Germany.
contact emailstephan.sieber@tum.de
lab head
Isabel Wilkinson
contact affiliationTechnical University of Munich
contact emailisabel.wilkinson@tum.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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