PXD036113

PXD036113 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling facilitates defining neighborhood proteome of photosystem II oxygen evolution complex in a model cyanobacterium
Description	APEX2-based proximity labeling coupled with mass spectrometry have great potential for spatiotemporal identification of proteins proximal to a protein complex of interest. Using this approach it is feasible to define the proteome neighborhood of important protein complexes in a popular photosynthetic model cyanobacterium Synechocystis sp. PCC6803 (hereafter named as Synechocystis). To this end, we developed a robust workflow for APEX2-based proximity labeling in Synechocystis, and used the workflow to identify proteins proximal to the photosystem II (PS II) oxygen evolution complex (OEC) through fusion APEX2 with a luminal OEC subunit, PsbO.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:02:32.796.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chengcheng Huang
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-16 13:58:56	ID requested
1	2023-02-17 01:45:01	announced
⏵ 2	2023-11-14 08:02:33	announced	2023-11-14: Updated project metadata.

Xiao Z, Huang C, Ge H, Wang Y, Duan X, Wang G, Zheng L, Dong J, Huang X, Zhang Y, An H, Xu W, Wang Y, Proximity Labeling Facilitates Defining the Proteome Neighborhood of Photosystem II Oxygen Evolution Complex in a Model Cyanobacterium. Mol Cell Proteomics, 21(12):100440(2022) [pubmed]

submitter keyword: Proximity labeling, Synechocystis,APEX, Proteomics, Cyanobacteria

Yingchun Wang
contact affiliation	State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Rd., Beijing 100101, China
contact email	ycwang@genetics.ac.cn
lab head
Chengcheng Huang
contact affiliation	University of Chinese Academy of Sciences
contact email	cc.huang@genetics.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD036113

PRIDE project URI

• PRIDE
  1. PXD036113
     1. Label: PRIDE project
     2. Name: Proximity labeling facilitates defining neighborhood proteome of photosystem II oxygen evolution complex in a model cyanobacterium