PXD036101 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | N‑Glycoproteomics Study of Putative N‑Glycoprotein Biomarkers of Drug Resistance in MCF‑7/ADR Cells |
Description | Currently, drug resistance of anti-cancer therapy has become the main cause of low survival rate and poor prognosis. Full understanding of drug resistance mechanisms is an urgent request for further development of anti-cancer therapy and improvement of prognosis. Here we present our N-glycoproteomics study of putative N-glycoprotein biomarkers of drug resistance in doxorubicin resistance breast cancer cell line michigan cancer foundation-7 (MCF-7/ADR) relative to parental michigan cancer foundation-7 (MCF-7) cells. Intact N-glycopeptides (IDs) from MCF-7/ADR and MCF-7 cells were enriched with zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC), labeled with stable isotopic diethylation (SIDE), and analyzed with C18-RPLC-MS/MS (HCD with stepped normalized collision energies); these IDs were identifed with database search engine GPSeeker, and the diferentially expressed intact N-glycopeptides (DEGPs) were quantifed with GPSeekerQuan. With target-decoy searches and control of spectrum-level FDR≤1%, 322 intact N-glycopeptides were identifed; these intact N-glycopeptides come from the combination of 249 unique peptide backbones (corresponding to 234 intact N-glycoproteins) and 90 monosaccharide compositions (corresponding to 248 putative N-glycosites). The sequence structures of 165 IDs were confrmed with structure-diagnostic fragment ions. With the criteria of observation at least twice among the three technical replicates,≥1.5-fold change and p value<0.05, 20 DEGPs were quantifed, where fve of them were up-regulated and 15 of them were down-regulated; the corresponding intact N-glycoproteins as putative markers of drug resistance were discussed. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-30 |
AnnouncementXML | Submission_2024-01-02_04:19:15.617.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yang hailun |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-16 13:42:04 | ID requested | |
⏵ 1 | 2024-01-02 04:19:15 | announced | |
Publication List
Keyword List
submitter keyword: MCF-7/ADR cells · MCF-7 cells · Drug resistance · Diferential N-glycosylation · N-glycoproteomics |
Contact List
Zhixin Tian |
contact affiliation | School of Chemical Science & Engineering, Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University |
contact email | zhixintian@tongji.edu.cn |
lab head | |
Yang hailun |
contact affiliation | Tongji University |
contact email | 1810917@tongji.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036101
- Label: PRIDE project
- Name: N‑Glycoproteomics Study of Putative N‑Glycoprotein Biomarkers of Drug Resistance in MCF‑7/ADR Cells