PXD036097

PXD036097 is an original dataset announced via ProteomeXchange.

Title	SWATH-MS demonstrates changes to protein expression due to a phase variable methyltransferase, ModA16, in Haemophilus influenzae biotype aegyptius
Description	Cytoplasmic DNA methyltransferases are often associated with restriction modification (R-M) systems in bacterial systems. Phase variable expression of these DNA methyltransferase results in epigenetic regulation of multiple genes as part of systems called phasevarions (phase-variable regulons). H. aegyptius is the causative agent of Brazilian Purpuric Fever (BPF), an invasive disease with high mortality, that sporadically manifests in children previously suffering conjunctivitis. We have recently described a previously unidentified allele of a phase variable Type III DNA methylstransferase in H. aegyptius, ModA16. When ModA16 is expressed, H. aegyptius strains exhibit differential methylation throughout the genome compared to when ModA16 is not expressed. This change in methylation results in altered gene expression and SWATH-MS was used to identify the resulting changes to protein expression. This dataset represents triplicate repeats from strains which express ModA16 (ON) and strains which do not (OFF).
HostingRepository	PRIDE
AnnounceDate	2023-11-15
AnnouncementXML	Submission_2023-11-14_17:29:25.932.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Greg Tram
SpeciesList	scientific name: Haemophilus influenzae biotype aegyptius; NCBI TaxID: 725;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-16 13:40:27	ID requested
⏵ 1	2023-11-14 17:29:26	announced

Dataset with its publication pending

submitter keyword: phasevarion, Haemophilus influenzae biogroup aegyptius,phase variation

John M Atack
contact affiliation	Institute for Glycomics, Griffith University, Gold Coast, Queensland 4215, Australia
contact email	j.atack@griffith.edu.au
lab head
Greg Tram
contact affiliation	Institute for Glycomics, Griffith University
contact email	g.tram@griffith.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD036097

PRIDE project URI

• PRIDE
  1. PXD036097
     1. Label: PRIDE project
     2. Name: SWATH-MS demonstrates changes to protein expression due to a phase variable methyltransferase, ModA16, in Haemophilus influenzae biotype aegyptius