PXD036078

PXD036078 is an original dataset announced via ProteomeXchange.

Title	C. Novyi's TcnA on HEp-2 cells, whole cell lysate Phosphoproteomics
Description	The effect of TcnA on target cells is largely unexplored. Therefore, we performed a comprehensive screening approach of TcnA treated HEp-2 cells and analyzed their proteome and their phosphoproteome using LC-MS-based methods. With this data-dependent acquisition (DDA) approach, 5,086 proteins and 9,427 phosphosites could be identified and quantified. Of these, 35 proteins were found to be significantly altered after toxin treatment, and 1,832 phosphosites were responsive to TcnA treatment. By analyzing the TcnA-induced proteomic effects of HEp-2 cells, 23 common signaling pathways were identified to be altered, including Actin Cytoskeleton Signaling, Epithelial Adherens Junction Signaling, and Signaling by Rho Family GTPases. All those pathways are also regulated after application of TcdA or TcdB of C. difficile. After TcnA treatment the regulation on phosphorylation level was much stronger compared to the proteome level, in terms of both strength of regulation and the number of regulated phosphosites. Interestingly, various signaling pathways such as Signaling by Rho Family GTPases or Integrin Signaling were activated on proteome level while being inhibited on phosphorylation level or vice versa as observed for Role of BRCA1 in DNA Damage Response. ZIP kinase, as well as Calmodulin-dependent protein kinases IV & II, were observed as activated while Aurora-A kinase and CDK kinases tended to be inhibited in cells treated with TcnA based on their substrate regulation pattern.
HostingRepository	PRIDE
AnnounceDate	2022-09-02
AnnouncementXML	Submission_2022-09-02_06:59:33.032.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036078
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Theresa Schweitzer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-16 07:00:53	ID requested
⏵ 1	2022-09-02 06:59:33	announced

Dataset with its publication pending

submitter keyword: C. novyi

Clostridium novyi, TcnA

LCGTs

C. difficile

TcdA

TcdB

proteomics

pathway analysis

phosphoproteomics

Andreas Pich
contact affiliation	Toxicology, ZFA Proteomics, Medical School Hannover, Germany
contact email	pich.andreas@mh-hannover.de
lab head
Theresa Schweitzer
contact affiliation	Toxikology
contact email	schweitzer.theresa@mh-hannover.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036078
     1. Label: PRIDE project
     2. Name: C. Novyi's TcnA on HEp-2 cells, whole cell lysate Phosphoproteomics