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PXD036077

PXD036077 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStrain- and cell type-specific influenza A virus (IAV)-host protein-protein interaction (PPI) network identified by affinity purification-mass spectrometry (AP-MS)
DescriptionInfluenza A Virus (IAV) is a recurring respiratory virus with antiviral therapies of limited use. Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses with three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we mapped 332 IAV-human protein-protein interactions and identified 13 IAV-modulated kinases. Whole exome sequencing of patients who experienced severe influenza revealed several genes, including the structural scaffold protein AHNAK, with predicted loss-of-function variants that were also identified in our proteomic analyses. Of our identified host factors, 54 significantly altered IAV infection upon siRNA knockdown, and two factors, COPB1 and AHNAK, were also essential for productive infection by SARS-CoV-2. Finally, 16 compounds targeting our identified host factors suppressed IAV replication, with three targeting ATP6V1A, CDK2 and FLT3 showing pan-antiviral activity across influenza and coronavirus families. This study provides a comprehensive network model of IAV infection in human cells, identifying functional host targets for pan-viral HDT. This project includes the AP-MS data; all global proteomic data (abundance and phosphorylation) has been submitted separately as its own dataset and has its own dataset identifier.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:57:47.615.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRobyn Kaake
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-16 06:30:54ID requested
12023-07-25 19:29:31announced
22024-10-22 05:57:48announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: virus-host, H5N1, Human, PPI, H1N1, A549, NHBE, H3N2, THP-1,AP-MS, Influenza A Virus
Contact List
Robyn Kaake
contact affiliationDept. Cellular Molecular Pharmacology, University of San Francisco (UCSF), USA
contact emailrobyn.kaake@gladstone.ucsf.edu
lab head
Robyn Kaake
contact affiliationDepartment of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA, USA
contact emailrobyn.kaake@gladstone.ucsf.edu
dataset submitter
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Dataset FTP location
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