PXD036068

PXD036068 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic analysis identifies molecular players and biological processes specific to SARS-CoV-2 exposure in endothelial cells
Description	In this study we investigated the global proteomic response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2. For this porpouse HUVECs were incubated with SARS-CoV-2 at a multiplicity of infection (MOI) of 1 and after different hours post-infection (hpi) samples were submitted to the 50% tissue-culture infective dose assay (TCID50), proteomic analysis and RT-qPCR measurements.To perform the relative quantitative label-free proteomic analysis, cells exposed to SARS-CoV-2 or MOCK were submitted to protein extraction, trypsin digestion and the tryptic peptides were analyzed in a Q-Exactive mass spectrometer. Data analysis of the differently abundant proteins of each cell model revealed distinct cell responses.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:34:34.145.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dilza Trevisan Silva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-16 05:22:44	ID requested
1	2022-11-29 04:44:17	announced
⏵ 2	2023-11-14 07:34:36	announced	2023-11-14: Updated project metadata.

Publication List

de Melo TC, Trevisan-Silva D, Alvarez-Flores MP, Gomes RN, de Souza MM, Valerio HP, Oliveira DS, DeOcesano-Pereira C, Botosso VF, Calil Jorge SA, Schattner M, Gomez RM, Chudzinski-Tavassi AM, Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells. Int J Mol Sci, 23(18):(2022) [pubmed]

de Melo TC, Trevisan-Silva D, Alvarez-Flores MP, Gomes RN, de Souza MM, Valerio HP, Oliveira DS, DeOcesano-Pereira C, Botosso VF, Calil Jorge SA, Schattner M, Gomez RM, Chudzinski-Tavassi AM, Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells. Int J Mol Sci, 23(18):(2022) [pubmed]

Keyword List

ProteomeXchange project tag: Sars-cov-2, Covid-19
submitter keyword: SARS-CoV-2, Mass Spectrometry, Endothelial Cells, Proteomics, HUVEC

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2, Mass Spectrometry, Endothelial Cells, Proteomics, HUVEC

Contact List

Ana Marisa Chudzinski-Tavassi
contact affiliation	Centre of Excellence in New Target Discovery (CENTD), Butantan Institute, Sao Paulo, Brazil.
contact email	ana.chudzinski@butantan.gov.br
lab head
Dilza Trevisan Silva
contact affiliation	University of Virginia
contact email	dilzatrevisan@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD036068
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD036068

PRIDE project URI

Repository Record List

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