PXD036049

PXD036049 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics and phosphoproteomics analyse the effect of ocnus on Drosophila spermatogenesis
Description	For phophoproteomics, the nosGal4>w- (control) samples 1, 2, and 3 were labeled with iTRAQ tags 118, 119, and 121, respectively. Samples of nosGal4>ocn-hp 1, 2, and 3 were labeled with iTRAQ tags 115, 116, and 117, respectively. The labeled peptides were desalted and concentrated in Sep-Pack C18 Cartridges (Waters, Milford, MA, USA). Then, iTRAQ-labeled peptides were added into the freshly prepared TiO2 beads at peptides-to-beads ratio of 1 : 4 for phosphopeptide enrichment. The peptide-beads’ slurry was shaken on a rotator at 37 °C for 1 h and then centrifuged for 30 s and then washed. Phosphopeptides were finally eluted with elution buffer for further analysis.
HostingRepository	iProX
AnnounceDate	2022-08-13
AnnouncementXML	Submission_2023-05-29_20:00:06.897.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ya Zheng
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-14 19:20:18	ID requested
1	2022-08-14 19:20:43	announced
⏵ 2	2023-05-29 20:00:07	announced	2023-05-30: Update publication information.

Zheng Y, Mao B, Wang Q, Duan X, Chen MY, Shen W, Li C, Wang YF, Quantitative proteomics and phosphoproteomics reveal insights into mechanisms of ocnus function in Drosophila testis development. BMC Genomics, 24(1):283(2023) [pubmed]

submitter keyword: proteomics, phosphoproteomics, ocnus, Drosophila spermatogenesis

Yufeng Wang
contact affiliation	Central China Normal University
contact email	yfengw@ccnu.edu.cn
lab head
Ya Zheng
contact affiliation	Central China Normal University
contact email	zhengyahb@mail.ccnu.edu.cn
dataset submitter

iProX dataset URI