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PXD036019

PXD036019 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSubunit composition, molecular environment and activation of native TRPC channels encoded by their interactomes
DescriptionIn the mammalian brain TRPC channels, a family of Ca2+-permeable cation channels, are involved in a variety of processes from neuronal growth and synapse formation to transmitter release, synaptic transmission and plasticity. The molecular appearance and operation of native TRPC channels, however, remained poorly understood. Here we used high-resolution proteomics to show that TRPC channels in the rodent brain are macro-molecular complexes of more than 1 MDa in size that results from co-assembly of the tetrameric channel core with an ensemble of interacting proteins (interactome). The core(s) of TRPC1, 4, 5-containing channels are mostly heteromers with defined stoichiometries for each subtype, while TRPC 3, 6, 7 preferentially form homomers. In addition, TRPC1, 4, 5-channels may co-assemble with the metabotropic glutamate receptor mGluR1 thus guaranteeing both specificity and reliability of channel activation via the phospholipase-Ca2+ pathway. Our results unveil the subunit composition of native TRPC channels and resolve molecular details underlying their activation.
HostingRepositoryPRIDE
AnnounceDate2024-07-17
AnnouncementXMLSubmission_2024-07-17_07:32:14.967.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD036019
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexander Haupt
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-12 13:08:27ID requested
12024-07-17 07:32:15announced
Publication List
Kollewe A, Schwarz Y, Oleinikov K, Raza A, Haupt A, Wartenberg P, Wyatt A, Boehm U, Ectors F, Bildl W, Zolles G, Schulte U, Bruns D, Flockerzi V, Fakler B, Subunit composition, molecular environment, and activation of native TRPC channels encoded by their interactomes. Neuron, 110(24):4162-4175.e7(2022) [pubmed]
10.6019/PXD036019;
10.1016/j.neuron.2022.09.029;
Keyword List
submitter keyword: LC-MSMS,mouse, brain, rat, TRPC
Contact List
Prof. Dr. Bernd Fakler
contact affiliationInstitute of Physiology II Universität Freiburg Germany
contact emailbernd.fakler@physiologie.uni-freiburg.de
lab head
Alexander Haupt
contact affiliationUniversity of Freiburg, Germany
contact emailalexander.haupt@physiologie.uni-freiburg.de
dataset submitter
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Dataset FTP location
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