PXD036019 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Subunit composition, molecular environment and activation of native TRPC channels encoded by their interactomes |
Description | In the mammalian brain TRPC channels, a family of Ca2+-permeable cation channels, are involved in a variety of processes from neuronal growth and synapse formation to transmitter release, synaptic transmission and plasticity. The molecular appearance and operation of native TRPC channels, however, remained poorly understood. Here we used high-resolution proteomics to show that TRPC channels in the rodent brain are macro-molecular complexes of more than 1 MDa in size that results from co-assembly of the tetrameric channel core with an ensemble of interacting proteins (interactome). The core(s) of TRPC1, 4, 5-containing channels are mostly heteromers with defined stoichiometries for each subtype, while TRPC 3, 6, 7 preferentially form homomers. In addition, TRPC1, 4, 5-channels may co-assemble with the metabotropic glutamate receptor mGluR1 thus guaranteeing both specificity and reliability of channel activation via the phospholipase-Ca2+ pathway. Our results unveil the subunit composition of native TRPC channels and resolve molecular details underlying their activation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-17 |
AnnouncementXML | Submission_2024-07-17_07:32:14.967.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD036019 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alexander Haupt |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-12 13:08:27 | ID requested | |
⏵ 1 | 2024-07-17 07:32:15 | announced | |
Publication List
Kollewe A, Schwarz Y, Oleinikov K, Raza A, Haupt A, Wartenberg P, Wyatt A, Boehm U, Ectors F, Bildl W, Zolles G, Schulte U, Bruns D, Flockerzi V, Fakler B, Subunit composition, molecular environment, and activation of native TRPC channels encoded by their interactomes. Neuron, 110(24):4162-4175.e7(2022) [pubmed] |
10.6019/PXD036019; |
10.1016/j.neuron.2022.09.029; |
Keyword List
submitter keyword: LC-MSMS,mouse, brain, rat, TRPC |
Contact List
Prof. Dr. Bernd Fakler |
contact affiliation | Institute of Physiology II Universität Freiburg Germany |
contact email | bernd.fakler@physiologie.uni-freiburg.de |
lab head | |
Alexander Haupt |
contact affiliation | University of Freiburg, Germany |
contact email | alexander.haupt@physiologie.uni-freiburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036019
- Label: PRIDE project
- Name: Subunit composition, molecular environment and activation of native TRPC channels encoded by their interactomes