PXD036019

PXD036019 is an original dataset announced via ProteomeXchange.

Title	Subunit composition, molecular environment and activation of native TRPC channels encoded by their interactomes
Description	In the mammalian brain TRPC channels, a family of Ca2+-permeable cation channels, are involved in a variety of processes from neuronal growth and synapse formation to transmitter release, synaptic transmission and plasticity. The molecular appearance and operation of native TRPC channels, however, remained poorly understood. Here we used high-resolution proteomics to show that TRPC channels in the rodent brain are macro-molecular complexes of more than 1 MDa in size that results from co-assembly of the tetrameric channel core with an ensemble of interacting proteins (interactome). The core(s) of TRPC1, 4, 5-containing channels are mostly heteromers with defined stoichiometries for each subtype, while TRPC 3, 6, 7 preferentially form homomers. In addition, TRPC1, 4, 5-channels may co-assemble with the metabotropic glutamate receptor mGluR1 thus guaranteeing both specificity and reliability of channel activation via the phospholipase-Ca2+ pathway. Our results unveil the subunit composition of native TRPC channels and resolve molecular details underlying their activation.
HostingRepository	PRIDE
AnnounceDate	2024-07-17
AnnouncementXML	Submission_2024-07-17_07:32:14.967.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036019
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Haupt
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-12 13:08:27	ID requested
⏵ 1	2024-07-17 07:32:15	announced

Kollewe A, Schwarz Y, Oleinikov K, Raza A, Haupt A, Wartenberg P, Wyatt A, Boehm U, Ectors F, Bildl W, Zolles G, Schulte U, Bruns D, Flockerzi V, Fakler B, Subunit composition, molecular environment, and activation of native TRPC channels encoded by their interactomes. Neuron, 110(24):4162-4175.e7(2022) [pubmed]

10.6019/PXD036019;

10.1016/j.neuron.2022.09.029;

submitter keyword: LC-MSMS,mouse, brain, rat, TRPC

Prof. Dr. Bernd Fakler
contact affiliation	Institute of Physiology II Universität Freiburg Germany
contact email	bernd.fakler@physiologie.uni-freiburg.de
lab head
Alexander Haupt
contact affiliation	University of Freiburg, Germany
contact email	alexander.haupt@physiologie.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036019
     1. Label: PRIDE project
     2. Name: Subunit composition, molecular environment and activation of native TRPC channels encoded by their interactomes