PXD036009

PXD036009 is an original dataset announced via ProteomeXchange.

Title	N7-methylguanosine methylation of tRNAs regulates survival to stress in cancer
Description	Combining transcriptome-wide approaches to detect m7G RNA methylation, in vitro functional assays and Mettl1 knockout mouse models, we provide evidence that guanosine-7 tRNA methylation is required to protect tRNAs from cleavage in response to stress, leading to impaired regulation of protein synthesis. Loss of METTL1 and tRNA methylation sensitises cancer cells to stress, reducing tumour growth and increasing cytotoxic responses to convectional cancer treatments in vitro and in vivo. Our study uncovers the role of m7G methylation of tRNAs in stress responses and highlights the potential of targeting METTL1 to sensitise cancer cells to therapy.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_08:14:33.963.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036009
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mikel Azkargorta
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-12 12:36:32	ID requested
⏵ 1	2024-01-26 08:14:34	announced

10.6019/PXD036009;

10.1038/s41388-023-02825-0;

Garc, í, a-V, í, lchez R, A, ñ, azco-Guenkova AM, L, ó, pez J, Dietmann S, Tom, é M, Jimeno S, Azkargorta M, Elortza F, B, á, rcena L, Gonzalez-Lopez M, Aransay AM, S, á, nchez-Mart, í, n MA, Huertas P, Dur, á, n RV, Blanco S, N7-methylguanosine methylation of tRNAs regulates survival to stress in cancer. Oncogene, 42(43):3169-3181(2023) [pubmed]

submitter keyword: autophagy, 7-methylguanosine, synthetic lethality., prostate cancer, survival, tRNA fragments,Epitranscriptome, senescence, cytotoxic stress

Felix Elortza
contact affiliation	Proteomics Service Bizakia Tech. Park Build. 800 Derio 48160 (Spain)
contact email	felorza@cicbiogune.es
lab head
Mikel Azkargorta
contact affiliation	Proteomics Platform CIC bioGUNE
contact email	mazkargorta@cicbiogune.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD036009

PRIDE project URI

• PRIDE
  1. PXD036009
     1. Label: PRIDE project
     2. Name: N7-methylguanosine methylation of tRNAs regulates survival to stress in cancer