PXD035999

PXD035999 is an original dataset announced via ProteomeXchange.

Title	Dual inhibition of SHP2 and autophagy suppresses NF1-associated malignant peripheral nerve sheath tumors
Description	Malignant peripheral nerve sheath tumors (MPNSTs) are the leading cause of premature death for patients with Neurofibromatosis type 1 and no approved targeted therapies are available. Benign plexiform neurofibromas have been successfully treated with selumetinib, a MEK inhibitor, but after progression to MPNST, MEK inhibition alone is not effective. Frequently, adaptive responses to single agent targeted inhibitors invokes alterations in receptor tyrosine kinase expression and feedback regulation that leads to inhibitor bypass. Here, the effects of SHP099, an inhibitor of the protein-tyrosine phosphatase SHP2 (encoded by the PTPN11 gene) was tested alone and in combination with trametinib (MEKi) in an orthotopic implantation murine model of NF1 MPNST with defined genotype (NP, Nf1-/-;Trp53-/-). Kinase enrichment proteomic analysis was performed using tumor tissue from vehicle, SHP099, trametinib, or trametinib and SHP099 in combination for 5 or 21 days to evaluate the effects on the functional kinome.
HostingRepository	PRIDE
AnnounceDate	2024-11-20
AnnouncementXML	Submission_2024-11-20_08:51:49.140.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Angus
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-12 03:17:28	ID requested
⏵ 1	2024-11-20 08:51:49	announced

Dataset with its publication pending

submitter keyword: SHP099, therapeutics, patient-derived xenograft, kinase inhibitor, genetically engineered mouse model, hydroxychloroquine,Neurofibromatosis type 1, trametinib, kinome

Luis F. Parada
contact affiliation	Memorial Sloan Kettering Cancer Center
contact email	paradal@mskcc.org
lab head
Steven Angus
contact affiliation	Indiana University School of Medicine
contact email	sangus@iu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD035999

PRIDE project URI

• PRIDE
  1. PXD035999
     1. Label: PRIDE project
     2. Name: Dual inhibition of SHP2 and autophagy suppresses NF1-associated malignant peripheral nerve sheath tumors