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PXD035996

PXD035996 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleKCNQ1OT1 promotes genome-wide transposon repression by guiding RNA-DNA triplexes and HP1 binding
DescriptionTransposon (de)repression and heterochromatin reorganization are dynamically regulated during cell fate determination and are hallmarks of cellular senescence. However, whether they are sequence specifically regulated remains unknown. Here, we uncover the KCNQ1OT1 lncRNA, by sequence-specific Hoogsteen base-pairing with double-stranded genomic DNA via its repeat-rich region and binding to heterochromatin protein HP1⍺, guides, induces and maintains epigenetic silencing at specific repetitive DNA elements. Repressing KCNQ1OT1 or deleting its repeat-rich region reduces DNA methylation and H3K9me3 on KCNQ1OT1 targeted transponsons. Engineering a fusion KCNQ1OT1 with an ectopically targeting guiding triplex sequence induces de novo DNA methylation at the target site. Phenotypically, repressing KCNQ1OT1 induces senescence associated heterochromatin foci, transposon activation and retrotransposition, and cellular senescence, demonstrating an essential role of KCNQ1OT1 to safeguard against genome instability and senescence.
HostingRepositoryPRIDE
AnnounceDate2022-09-05
AnnouncementXMLSubmission_2022-09-04_19:53:17.050.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD035996
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJiyang LI
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-12 02:20:10ID requested
12022-09-04 19:53:17announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: KCNQ1OT1, heterochromatin, transposon, RNA-dsDNA triplex, senescence
Contact List
Jing-Dong J. Han
contact affiliationPeking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Center for Quantitative Biology (CQB), Peking University; Beijing 100871, China
contact emailjackie.han@pku.edu.cn
lab head
Jiyang LI
contact affiliationCAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences; 320 Yue Yang Road, Shanghai, 200031, China University of Chinese Academy of Sciences; Beijing 100049, China School of Life Science and Technology, ShanghaiTech University; Shanghai, 201210, China
contact emailmeng_ljy@126.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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