PXD035992

PXD035992 is an original dataset announced via ProteomeXchange.

Title	Systematic evaluation of protein synthesis inhibition through global quantification of newly synthesized protiens
Description	Manipulating protein synthesis is commonly applied in bio-related research to uncover protein functions and cellular activities. Multiple inhibitors with distinct mechanisms are widely used, but it is extraordinarily challenging to systematically determine and evaluate their inhibition efficiencies, especially for individual proteins. Newly synthesized proteins are direct products of protein synthesis, and thus their comprehensive and quantitative analysis provides a unique opportunity to study protein synthesis inhibition. Here, we systematically investigate and evaluate popular inhibitors through global quantification of nascent proteins in several types of human cells. The same inhibitor results in dramatic differences in the inhibition efficiencies for different proteins, and each inhibitor exhibits unique synthesis inhibition preference. Overall, nucleolar and ribosomal proteins have relatively higher inhibition efficiencies, while the efficiencies of proteins in the peroxisome and the Golgi are lower. The sensitivity of proteins to the inhibition can reflect the characteristics of each cell type. Moreover, proteins generally resistant to the inhibition have lower translation efficiency, among which chaperones are overrepresented. Systematic evaluation of protein synthesis inhibition aids in better understanding protein synthesis regulation and provides valuable information to the biology and biomedicine communities.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:05.126.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kejun Yin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-11 03:09:22	ID requested
1	2023-05-24 15:13:53	announced
⏵ 2	2023-11-14 08:58:05	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: bioorhogonal chemistry

protein synthesis inhibtion

protein inhibition efficiency

mass spectromety

multiplexed proteomics

newly synthesized proteins

Ronghu Wu
contact affiliation	Department of Chemistry and Biochemistry, Georgia Insititute of Technology
contact email	ronghu.wu@chemistry.gatech.edu
lab head
Kejun Yin
contact affiliation	Georgia Institute of Technology
contact email	kyin49@gatech.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035992
     1. Label: PRIDE project
     2. Name: Systematic evaluation of protein synthesis inhibition through global quantification of newly synthesized protiens