PXD035986 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic evaluation of human osteoblast responses to titanium implants over time |
Description | Titanium is widely used in bone prostheses due to its excellent biocompatibility and osseointegration capacity. To understand the effect of sandblasted acid-etched (SAE) Ti implants on the biological responses of human osteoblast (HOb), their proteomic profiles were analysed using nLC-MS/MS. The cells were cultured with the implant materials, and 2544 distinct proteins were detected in samples taken after 1, 3 and 7 days. Comparative analyses of proteomic data were performed using Perseus software. The expression of proteins related to EIF2, mTOR, insulin-secretion and IGF pathways showed marked differences in cells grown with SAE-Ti in comparison with cells cultured without Ti. Moreover, the proteomic profiles obtained with SAE-Ti were compared over time. The affected proteins were related to adhesion, immunity, oxidative stress, coagulation, angiogenesis, osteogenesis and extracellular matrix formation functions. The proliferation, mineralisation and osteogenic gene expression in HObs cultured with SAE-Ti were characterised in vitro. The results showed that the osteoblasts exposed to this material increase their mineralisation rate and expression of COLI, RUNX2, SP7, CTNNB1, CAD13, IGF2, MAPK2 and mTOR. Overall, the observed proteomic profiles can explain the SAE-Ti osteogenic properties, widening our knowledge of key signalling pathways taking part in the early stages of the osseointegration process in this type of implantations. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:43:08.155.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mikel Azkargorta |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-11 03:04:00 | ID requested | |
1 | 2023-03-11 06:04:07 | announced | |
⏵ 2 | 2023-11-14 08:43:16 | announced | 2023-11-14: Updated project metadata. |
Publication List
Romero-Gavil, á, n F, Cerqueira A, Garc, í, a-Arn, á, ez I, Azkargorta M, Elortza F, Gurruchaga M, Go, ñ, i I, Suay J, Proteomic evaluation of human osteoblast responses to titanium implants over time. J Biomed Mater Res A, 111(1):45-59(2023) [pubmed] |
Keyword List
submitter keyword: Titanium, human osteoblasts, label free |
Contact List
Felix Elortza |
contact affiliation | Proteomics Service CIC bioGUNE Bizkaia Tech. Park Build. 800 48160 Derio (Spain) |
contact email | felortza@cicbiogune.es |
lab head | |
Mikel Azkargorta |
contact affiliation | Proteomics Platform CIC bioGUNE |
contact email | mazkargorta@cicbiogune.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035986
- Label: PRIDE project
- Name: Proteomic evaluation of human osteoblast responses to titanium implants over time