PXD035955

PXD035955 is an original dataset announced via ProteomeXchange.

Title	Apocarotenoids are allosteric effectors of a dimeric plant glycosyltransferase involved in plant defense and lignin formation
Description	Glycosyltransferases are Nature’s versatile tools to tailor the functionalities of proteins, carbohydrates, lipids, and small molecules by transferring sugars, while C13-apocarotenoids are oxidative degradation products of carotenoids/xanthophylls that fulfil multiple physiological functions. Here, we show that C13-apocarotenoids also interact with the plant glycosyltransferase NbUGT72AY1, ultimately reducing its inherent UDP-α-D-glucose glucohydrolase activity and increasing its catalytic activity for productive substrates such as hydroxycoumarins, which are natural plant protective agents. In contrast, C13-apocarotenoids show no effect on the catalytic activity of the enzyme toward monolignol lignin precursors. Hydrogen deuterium exchange mass spectrometry revealed opposite conformational changes near the catalytic site in the presence of substrates and modulators and studies in tobacco plants confirmed increased glycosylation activity from apocarotenoid supplementation. The interaction of two plant protection mechanisms and the function of apocarotenoids as allosteric enhancers of catalytic proteins has significant implications for potential applications of these multifunctional natural products in agriculture as well as in the pharmaceutical industry.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:36.249.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-10 09:31:45	ID requested
⏵ 1	2023-11-14 08:44:41	announced

Sun G, Liao J, Kurze E, Hoffmann TD, Steinchen W, McGraphery K, Habegger R, Marek L, Catici DAM, Ludwig C, Jing T, Hoffmann T, Song C, Schwab W, Apocarotenoids are allosteric effectors of a dimeric plant glycosyltransferase involved in defense and lignin formation. New Phytol, 238(5):2080-2098(2023) [pubmed]

submitter keyword: Phytoalexin, Activation,Glycosyltransferase, Ionol, Lignin, Apocarotenoids

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS) Technische Universität München (TUM) Gregor-Mendel-Straße 4 85354 Freising GERMANY
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035955
     1. Label: PRIDE project
     2. Name: Apocarotenoids are allosteric effectors of a dimeric plant glycosyltransferase involved in plant defense and lignin formation