PXD035952

PXD035952 is an original dataset announced via ProteomeXchange.

Title	Uncovering the protein alterations in anther tissues of three contrasting rice types in response to short-term and long-term heat stress conditions
Description	Heat stress drastically affects anther tissues resulting in poor plant fertility, necessitating an urgent need to determine the key proteins associated in the response of anthers to heat stress. Employing quantitative proteome analysis, we identified several cultivar-specific protein alterations in anthers of Moroberekan (Japonica, heat sensitive), IR64 (Indica, moderately heat tolerant), and Nagina 22 (Aus, heat tolerant) rice types following short-term (ST_HS; one cycle of 42°C, 4 hours before anthesis) and long-term (LT_HS; 6 cycles of 38°C, 6 hours before anthesis) heat treatments. The protein numbers were higher in Nagina 22 than IR 64 and Moroberekan anthers under unstressed and heat stress. The proteins upregulated in long-term heat stress in Nagina 22 were enriched in biological processes related to unfolded protein binding and carboxylic acid metabolism, including amino acid metabolism. In short-term heat stress, Nagina 22 anthers were enriched in proteins associated with vitamin E biosynthesis while proteins downregulated were related to ribosomal proteins. The expression of Hsp20, DnaJ, and Hsp101 was predominantly observed in Nagina 22. Overall, the heat response in Nagina 22 was associated with its capacity for adequate metabolic control and cellular homeostasis which may be critical for its higher reproductive thermotolerance
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:17:06.264.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Palak Chaturvedi
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-10 09:30:19	ID requested
1	2023-02-17 04:03:26	announced
⏵ 2	2023-11-14 08:17:07	announced	2023-11-14: Updated project metadata.

Kumar R, Ghatak A, Goyal I, Sarkar NK, Weckwerth W, Grover A, Chaturvedi P, Heat-induced proteomic changes in anthers of contrasting rice genotypes under variable stress regimes. Front Plant Sci, 13():1083971(2022) [pubmed]

submitter keyword: Nagina 22 rice, IR64 rice, Heat stress,Anthers, Proteomics, LC-MS/MS, Moroberekan rice, Reproductive thermotolerance

Palak Chaturvedi
contact affiliation	University of Vienna
contact email	palak.chaturvedi@univie.ac.at
lab head
Palak Chaturvedi
contact affiliation	University of Vienna
contact email	palak.chaturvedi@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035952
     1. Label: PRIDE project
     2. Name: Uncovering the protein alterations in anther tissues of three contrasting rice types in response to short-term and long-term heat stress conditions