PXD035950

PXD035950 is an original dataset announced via ProteomeXchange.

Title	Developmental Dynamics of RNA Translation in the Human Brain
Description	The precise regulation of gene expression is fundamental to neurodevelopment, plasticity, and cognitive function. While several studies have profiled transcription in the developing human brain, there is a gap in our understanding of accompanying translational regulation. We performed ribosome profiling on 73 human prenatal and adult cortex samples. We characterized the translational regulation of annotated open reading frames (ORFs) and identified thousands of previously unknown translation events, including small ORFs that give rise to human- and/or brain-specific microproteins, many of which we independently verified using proteomics. Ribosome profiling in stem cell-derived human neuronal cultures corroborated these findings and revealed that several neuronal activity-induced non-coding RNAs encode previously undescribed microproteins. Physicochemical analysis of brain microproteins identified a class of proteins that contain arginine-glycine-glycine (RGG) repeats and thus may be regulators of RNA metabolism. This resource expands the known translational landscape of the human brain and illuminates previously unknown brain-specific protein products.
HostingRepository	PRIDE
AnnounceDate	2022-09-29
AnnouncementXML	Submission_2022-09-29_01:48:50.268.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Erin Duffy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-10 09:22:51	ID requested
⏵ 1	2022-09-29 01:48:50	announced

Dataset with its publication pending

submitter keyword: human, brain, microprotein, development

Michael E. Greenberg
contact affiliation	Harvard Medical School Department of Neurobiology, Boston, MA, USA
contact email	michael_greenberg@hms.harvard.edu
lab head
Erin Duffy
contact affiliation	Harvard Medical School
contact email	erin_duffylacy@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD035950

PRIDE project URI

• PRIDE
  1. PXD035950
     1. Label: PRIDE project
     2. Name: Developmental Dynamics of RNA Translation in the Human Brain