PXD035945

PXD035945 is an original dataset announced via ProteomeXchange.

Title	Analysis of prolyl 4-hydroxylation of P4ha1 KO MEFs and P4ha2 KO mouse kidneys
Description	We established a mouse embryonic fibroblast (MEF) cell line derived from mice that have a genetic ablation of of P4ha1. Type I collagen produced by these cells in presence of ascorbate was collected and partially purified by precipitation. Site specific hydroxylation analysis was performed using mass spectrometry. In another setup, type IV collagen from kidneys of P4ha2-/- mice was partially purified using pepsin digestion in acetic acid. Gel bands corresponding type IV collagens were cut and analyzed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2023-12-15
AnnouncementXML	Submission_2023-12-15_03:23:16.193.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pekka Rappu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	O5-galactosyl-L-hydroxylysine; O5-glucosylgalactosyl-L-hydroxylysine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-10 09:21:03	ID requested
⏵ 1	2023-12-15 03:23:16	announced

10.1016/J.MATBIO.2023.12.001;

submitter keyword: mouse, prolyl 4-hydroxylation, collagen

Jyrki Heino
contact affiliation	Department of Life Technologies, University of Turku, Turku, Finland
contact email	jyrki.heino@utu.fi
lab head
Pekka Rappu
contact affiliation	University of Turku
contact email	pekka.rappu@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035945
     1. Label: PRIDE project
     2. Name: Analysis of prolyl 4-hydroxylation of P4ha1 KO MEFs and P4ha2 KO mouse kidneys