PXD035941

PXD035941 is an original dataset announced via ProteomeXchange.

Title	Cryo-electron microscopy unravels microtubule inner protein networks reinforcing mammalian sperm flagella
Description	To find and fuse with the egg, mammalian sperm must complete an arduous voyage through the female reproductive tract. This odyssey is powered by the sperm tail, a specialized motile cilium. Mammalian sperm tails are reinforced at the molecular scale with sperm-specific microtubule inner proteins (sperm-MIPs), but the identities of these sperm-MIPs are unknown. Here, we report high-resolution cryo-electron microscopy structures of bovine sperm doublet microtubules (DMTs), allowing us to identify many sperm-MIPs. We also resolve structures of singlet MTs in the endpiece, revealing MIPs shared between singlet and doublet MTs. We demonstrate that at least two sperm-MIPs bind and stabilize MTs in vitro. Our structures shed light on ciliary diversity across cell types and provide structural frameworks for understanding molecular underpinnings of male infertility
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:44:26.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Riccardo Zenezini Chiozzi
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-10 09:14:57	ID requested
1	2023-06-20 11:29:44	announced
⏵ 2	2023-11-14 06:44:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: EPIC-XS

submitter keyword: Sperm, proteomics,Crosslinking MS, tubulin, DSSO

Albert Heck
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, 3584CH Utrecht, Netherlands. Netherlands Proteomics Center, 3584CH Utrecht, Netherlands.
contact email	a.j.r.heck@uu.nl
lab head
Riccardo Zenezini Chiozzi
contact affiliation	UCL Mass Spectrometry Science Technology Platform, Division of Biosciences, University College London, London
contact email	r.zenezinichiozzi@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD035941

PRIDE project URI

• PRIDE
  1. PXD035941
     1. Label: PRIDE project
     2. Name: Cryo-electron microscopy unravels microtubule inner protein networks reinforcing mammalian sperm flagella