PXD035934 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mechanism of client kinase dephosphorylation by the HSP90-CDC37-PP5 complex |
Description | Activation of client protein kinases by the HSP90 molecular chaperone system is affected by phosphorylation at multiple sites on HSP90, on the kinase specific co-chaperone CDC37, and the client itself. Removal of regulatory phosphorylation from client kinases and release from the HSP90-CDC37 system depends on a Ser/Thr phosphatase PP5, which associates with HSP90 via its N-terminal TPR domain. Here we present the cryoEM structure of the oncogenic client kinase BRAFV600E bound to HSP90-CDC37, and structures of complexes of PP5 with that. Together with proteomic analysis of its phosphatase activity, our results reveal how PP5 is activated by recruitment to HSP90 complexes to dephosphorylate client proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:40:33.849.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Graeme Benstead-Hume |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-10 09:03:32 | ID requested | |
1 | 2023-03-11 02:19:11 | announced | |
⏵ 2 | 2023-11-14 08:40:34 | announced | 2023-11-14: Updated project metadata. |
Publication List
Oberoi J, Guiu XA, Outwin EA, Schellenberger P, Roumeliotis TI, Choudhary JS, Pearl LH, HSP90-CDC37-PP5 forms a structural platform for kinase dephosphorylation. Nat Commun, 13(1):7343(2022) [pubmed] |
Keyword List
submitter keyword: m6a methylation, neuronal differentiation, IMP1 regulation |
Contact List
Jyoti Choudhary |
contact affiliation | Functional Proteomics, ICR |
contact email | jyoti.choudhary@icr.ac.uk |
lab head | |
Graeme Benstead-Hume |
contact affiliation | ICR |
contact email | ghume@icr.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035934
- Label: PRIDE project
- Name: Mechanism of client kinase dephosphorylation by the HSP90-CDC37-PP5 complex