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PXD035934

PXD035934 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMechanism of client kinase dephosphorylation by the HSP90-CDC37-PP5 complex
DescriptionActivation of client protein kinases by the HSP90 molecular chaperone system is affected by phosphorylation at multiple sites on HSP90, on the kinase specific co-chaperone CDC37, and the client itself. Removal of regulatory phosphorylation from client kinases and release from the HSP90-CDC37 system depends on a Ser/Thr phosphatase PP5, which associates with HSP90 via its N-terminal TPR domain. Here we present the cryoEM structure of the oncogenic client kinase BRAFV600E bound to HSP90-CDC37, and structures of complexes of PP5 with that. Together with proteomic analysis of its phosphatase activity, our results reveal how PP5 is activated by recruitment to HSP90 complexes to dephosphorylate client proteins.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:40:33.849.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGraeme Benstead-Hume
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-10 09:03:32ID requested
12023-03-11 02:19:11announced
22023-11-14 08:40:34announced2023-11-14: Updated project metadata.
Publication List
Oberoi J, Guiu XA, Outwin EA, Schellenberger P, Roumeliotis TI, Choudhary JS, Pearl LH, HSP90-CDC37-PP5 forms a structural platform for kinase dephosphorylation. Nat Commun, 13(1):7343(2022) [pubmed]
Keyword List
submitter keyword: m6a methylation, neuronal differentiation, IMP1 regulation
Contact List
Jyoti Choudhary
contact affiliationFunctional Proteomics, ICR
contact emailjyoti.choudhary@icr.ac.uk
lab head
Graeme Benstead-Hume
contact affiliationICR
contact emailghume@icr.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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