PXD035923

PXD035923 is an original dataset announced via ProteomeXchange.

Title	Ribosome r-protein content during stationary phase
Description	The aim of this study was to determine the fate of ribosomes and r-proteins. In this respect, SILAC (Stable Isotope Labeled Amino acids in cell Culture) based experimental approach was used (Fig. 1). E.coli cells were grown in MOPS medium supplemented with “heavy” labeled arginine (Arg10) and lysine (Lys8). At the mid-log phase, the culture was further supplemented with a 20-fold molar excess of “light” unlabeled arginine (Arg0) and lysine (Lys0), divided into 8 aliquots, and grown for 14 days. Cell samples were collected at day one (24h), day two (48h), and subsequently in 48h intervals over the following 12 days. The ribosome particles were isolated using sucrose gradient centrifugation. the quantities of r-proteins in the 70S ribosome fraction were determined using SILAC based LC-MS/MS and normalized to the corresponding values of day one.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:46:44.714.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kaspar Reier
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	6x(13)C; 6x(13)C; (4,4,5,5-(2)H4)-L-lysine; monohydroxylated residue; 6x(13)C labeled L-arginine; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-10 08:01:37	ID requested
⏵ 1	2024-10-07 13:46:45	announced

Reier K, Lahtvee PJ, Liiv A, Remme J, A Conundrum of r-Protein Stability: Unbalanced Stoichiometry of r-Proteins during Stationary Phase in Escherichia coli. mBio, 13(5):e0187322(2022) [pubmed]

10.1128/mbio.01873-22;

submitter keyword: Ribosome, ribosomal proteins, proteome, E.coli

Tiina Tamm
contact affiliation	Chair of Molecular Biology Institute of Cellular and Molecular Biology Estonia
contact email	ttamm@ut.ee
lab head
Kaspar Reier
contact affiliation	University of Tartu
contact email	kaspar.reier@ut.ee
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035923
     1. Label: PRIDE project
     2. Name: Ribosome r-protein content during stationary phase