PXD035915

PXD035915 is an original dataset announced via ProteomeXchange.

Title	Metaproteomic and proteomic characterization of human bile of gallstone disease reveals the interplay between host and the microbiota
Description	Cholesterol gallstone is a common disease, but the pathogenesis of gallstone remains a mystery. The pathogenetic factors of gallstone disease include a genetic background, cholesterol condensation and bile supersaturation, while the risk factors may include fatty liver, diabetes, obesity and microbiome dysbiosis. Dysbiosis refers to altered bacterial composition and abundance, which is found to be associated with the pathogenesis of variety of diseases. Mass spectrometry-based proteomics emerges as a powerful tool to identify protein expressions of human samples. A number of proteomic studies have been performed to analyze human protein contents of bile samples. All these studies focus on the analysis of human proteins, and no study to date has been performed to analyze the protein expression of microbiome in bile samples associated with gallstone disease. In current study, we performed a label-free metaproteomic analysis of gallbladder bile samples from cholesterol gallstone disease patients and normal individuals. Maxquant was used in the comprehensive database searching to infer protein groups with quantitation. We attempt to investigate the dysbiosis changes, potential virulence factor of microbiome and host immune responses which contribute to gallstone formation and cholelithiasis.
HostingRepository	iProX
AnnounceDate	2022-08-10
AnnouncementXML	Submission_2024-02-26_19:43:09.378.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	methionine oxidation with neutral loss of 64 Da; acetylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-09 22:50:57	ID requested
1	2023-01-03 01:46:31	announced
⏵ 2	2024-02-26 19:43:10	announced	2024-02-27: Update information.

Yang XT, Wang J, Jiang YH, Zhang L, Du L, Li J, Liu F, Insight into the mechanism of gallstone disease by proteomic and metaproteomic characterization of human bile. Front Microbiol, 14():1276951(2023) [pubmed]

submitter keyword: Bile, Metaproteomics, Gallstone, Microbiome, Bacteria, Proteomics, Cholecystitis

Feng Liu
contact affiliation	Institutes of Biomedical of Sciences, Fudan University
contact email	liuf@fudan.edu.cn
lab head
Jie Wang
contact affiliation	Institutes of Biomedical of Sciences, Fudan University
contact email	tlwj1949@163.com
dataset submitter

iProX dataset URI