PXD035903

PXD035903 is an original dataset announced via ProteomeXchange.

Title	Hepatitis C virus induces HuR relocalization by coordinating PKC-δ and AMPK-α function
Description	Huh7.5 cells were transfected with HCV-JFH1 RNAand harvested after 48h of transfection. Total proteins were extracted in IP lysis buffer (Pierce, Thermo Scientific) containing 1× halt-protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, USA) with intermediate vortexing followed by mild sonication. The lysate was centrifuged at 14,000 × g for 30 min at 4 °C. The supernatant was quantified by the BCA method (Thermo Fisher Scientific, USA). An equivalent amount (4 mg) of proteins from each condition was incubated with 3g of anti-HuR antibody for overnight at 4 °C. The immunocomplex was captured by using protein G Sepharose 4 fast flow beads (17-0618-01/ GE Healthcare). The immunoprecipitated complex was washed two times with IP lysis buffer and one time with mili-Q. Bound protein complexes were eluted in 50 µl SDS-PAGE 2X Laemmli sample buffer. The samples were resolved on 12% SDS–PAGE and stained with Coomassie stain (0.1% w/v). The gel was subjected to further in-gel mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:27.461.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TUSHAR KANTI MAITI
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-08 10:50:49	ID requested
1	2023-09-15 21:36:46	announced
⏵ 2	2023-11-14 08:53:28	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: AMPK in virus infection,Hepatitis C virus, HuR phosphorylation, HuR translocation, PKC-δ in virus infection

Dr. Tushar K Maiti
contact affiliation	Regional Centre for Biotechnology
contact email	tkmaiti@rcb.res.in
lab head
TUSHAR KANTI MAITI
contact affiliation	Regional Centre for Biotechnology
contact email	tkmaiti@rcb.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD035903

PRIDE project URI

• PRIDE
  1. PXD035903
     1. Label: PRIDE project
     2. Name: Hepatitis C virus induces HuR relocalization by coordinating PKC-δ and AMPK-α function