PXD035900

PXD035900 is an original dataset announced via ProteomeXchange.

Title	Global Protein Abundance and Phosphorylation Analyses of Influenza A Virus (IAV)-Infected Human Cells
Description	Influenza A Virus (IAV) is a recurring respiratory virus with antiviral therapies of limited use. Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses with three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we mapped 332 IAV-human protein-protein interactions and identified 13 IAV-modulated kinases. Whole exome sequencing of patients who experienced severe influenza revealed several genes, including the structural scaffold protein AHNAK, with predicted loss-of-function variants that were also identified in our proteomic analyses. Of our identified host factors, 54 significantly altered IAV infection upon siRNA knockdown, and two factors, COPB1 and AHNAK, were also essential for productive infection by SARS-CoV-2. Finally, 16 compounds targeting our identified host factors suppressed IAV replication, with three targeting ATP6V1A, CDK2 and FLT3 showing pan-antiviral activity across influenza and coronavirus families. This study provides a comprehensive network model of IAV infection in human cells, identifying functional host targets for pan-viral HDT. This project includes the global proteomic data (abundance and phosphorylation), the AP-MS data has been submitted separately as its own dataset and has its own dataset identifier.
HostingRepository	PRIDE
AnnounceDate	2023-07-26
AnnouncementXML	Submission_2023-07-25_20:49:51.331.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	RobynKaake
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-08 10:21:26	ID requested
⏵ 1	2023-07-25 20:49:51	announced

Dataset with its publication pending

submitter keyword: Human, H5N1, H1N1, protein abundance, phosphorylation, NHBE, H3N2, THP-1, infection, Influenza A Virus

RobynKaake
contact affiliation	Dept. Cellular Molecular Pharmacology, University of California - San Francisco (UCSF), USA
contact email	robyn.kaake@gladstone.ucsf.edu
lab head
RobynKaake
contact affiliation	Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA, USA
contact email	robyn.kaake@gladstone.ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD035900

PRIDE project URI

• PRIDE
  1. PXD035900
     1. Label: PRIDE project
     2. Name: Global Protein Abundance and Phosphorylation Analyses of Influenza A Virus (IAV)-Infected Human Cells