PXD035897

PXD035897 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteome dataset profiling UBC4 and UBC5 deletion strains in Saccharomyces cerevisiae
Description	The Ubiquitin-Proteasome System (UPS) regulates many cellular processes in eukaryotic cells. Ubiquitylation by the UPS mainly directs proteins to proteasomal degradation, but it can also have non-degradative functions, such as regulating protein activity or localization. The small protein ubiquitin is conjugated to its substrates via a cascade of E1-E2-E3 enzymes. Dysregulation of the UPS has been implicated in the genesis and progression of many diseases, such as neurodegenerative diseases and cancer; thus, the UPS components are attractive targets for developing pharmaceutical drugs. E2s, or ubiquitin conjugating enzymes, are central players of the UPS. E2s function in tandem with specific ubiquitin ligases (E3s) to transfer ubiquitin to substrates. Here, we present the first proteome stability analysis of two closely related ubiquitin conjugating enzymes, UBC4 and UBC5, in S. cerevisiae. These two E2s are nearly identical, having 92% sequence identity and differing by only 11 amino acid residues. This dataset is of broad interest because higher eukaryotes express ubiquitin conjugating enzymes that are analogous to the yeast UBC4/5.
HostingRepository	PRIDE
AnnounceDate	2022-09-02
AnnouncementXML	Submission_2022-09-02_11:54:06.132.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-08 10:20:26	ID requested
⏵ 1	2022-09-02 11:54:06	announced

Dataset with its publication pending

submitter keyword: Ubiquitin proteasome system, protein stability, yeast, TMT, isobaric tagging

Joao A. Paulo
contact affiliation	Department of Cell Biology Harvard Medical School Boston, MA 02115, USA
contact email	joao_paulo@post.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035897
     1. Label: PRIDE project
     2. Name: Quantitative proteome dataset profiling UBC4 and UBC5 deletion strains in Saccharomyces cerevisiae