PXD035893

PXD035893 is an original dataset announced via ProteomeXchange.

Title	Multiomic analysis of Cohesin reveals that ZBTB transcription factors contribute to chromatin interactions
Description	One bottleneck in understanding the principles of 3D chromatin structures is caused by the paucity of known regulators. Cohesin is essential for 3D chromatin organization, and its interacting partners are candidate regulators. Here, we performed proteomic profiling of the Cohesin in chromatin and identified transcription factors, RNA-binding proteins, and chromatin regulators associated with Cohesin. Acute protein degradation followed by time-series genomic binding quantitation and BAT Hi-C analysis were conducted, and the results showed that the transcription factor ZBTB21 contributes to Cohesin chromatin binding, 3D chromatin interactions and transcriptional repression. Strikingly, multiomic analyses revealed that the other four ZBTB factors interacted with Cohesin, and double degradation of ZBTB21 and ZBTB7B led to a further decrease in Cohesin chromatin occupancy. We propose that multiple ZBTB transcription factors orchestrate the chromatin binding of Cohesin to regulate chromatin interactions, and we provide a catalog of many additional proteins associated with Cohesin that warrant further investigation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:36.050.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	RUI WANG
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-08 10:18:25	ID requested
1	2023-06-05 01:00:26	announced
⏵ 2	2023-11-14 08:48:41	announced	2023-11-14: Updated project metadata.

Wang R, Xu Q, Wang C, Tian K, Wang H, Ji X, Multiomic analysis of cohesin reveals that ZBTB transcription factors contribute to chromatin interactions. Nucleic Acids Res, 51(13):6784-6805(2023) [pubmed]

submitter keyword: Human,CH12F3,label-free,Mouse,HEK293Ts,ChIP-MS,B16,mESCs

Xiong Ji
contact affiliation	School of life science, Peking University,Beijing
contact email	xiongji@pku.edu.cn
lab head
RUI WANG
contact affiliation	PEKING University
contact email	wyandpwl@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD035893

PRIDE project URI

• PRIDE
  1. PXD035893
     1. Label: PRIDE project
     2. Name: Multiomic analysis of Cohesin reveals that ZBTB transcription factors contribute to chromatin interactions