PXD035886 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cellular heterogeneity and therapeutic response profiling of IDH-wildtype glioma stem cell cultures |
| Description | Gliomas are the most common type of primary malignant adult brain tumor. They appear to originate from neuroglial stem or progenitor cells, and are therapeutically challenging due to an invasive growth pattern and the absence of effective therapies. We have analyzed cellular, molecular and proteomic features and defined the therapeutic response profiles of four IDH1-wildtype glioma stem cell (GSC) cultures. All four GSC cultures were established from Grade IV glioblastoma (GBM) surgical resection tissue, can be continuously propagated and are highly enriched for stem/tumor repopulating cells. Integrated genomic and proteomic analysis of all four cultures was performed, together with use of a dual molecular bar-coding strategy to assess GSC population heterogeneity and the response to ionizing radiation. These well-characterized, bar-coded GSC cultures provide an experimentally tractable resource for investigating glioma biology, and to use to identify new and potentially more effective GBM therapies and treatment regimens. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-02 |
| AnnouncementXML | Submission_2026-02-01_20:33:37.258.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD035886 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Mario Leutert |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2022-08-08 09:51:40 | ID requested | |
| ⏵ 1 | 2026-02-01 20:33:38 | announced | |
Publication List
| 10.6019/PXD035886; |
| Chambwe N, Kennedy SR, Kohrn BF, Lazarchuk P, Leutert M, Qin G, Tercan B, Sanchez-Contreras M, Tang W, Graber JJ, Paddison PJ, Vill, é, n J, Shmulevich I, Monnat RJ, glioma stem cell cultures. Sci Rep, 16(1):3177(2025) [pubmed] |
| 10.1038/s41598-025-33082-8; |
Keyword List
| submitter keyword: Cancer, Stem Cell, Phopshoproteomics,Glioblastoma, Tumor |
Contact List
| Judit Villen |
|---|
| contact affiliation | Department of Genome Sciences, University of Washington, Seattle, USA |
| contact email | jvillen@uw.edu |
| lab head | |
| Mario Leutert |
|---|
| contact affiliation | Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA |
| contact email | marioleutert@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD035886 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035886
- Label: PRIDE project
- Name: Cellular heterogeneity and therapeutic response profiling of IDH-wildtype glioma stem cell cultures
