PXD035866

PXD035866 is an original dataset announced via ProteomeXchange.

Title	Targeted protein degradation reveals Pol II heterogeneity and functional diversity
Description	RNA polymerase II (Pol II) subunits are thought to be involved in various transcription-associated processes, but it is unclear whether they play different regulatory roles in modulating gene expression. Here, we performed nascent and mature transcript sequencing after the acute degradation of 12 mammalian Pol II subunits and profiled their genomic binding sites and protein interactomes to dissect their molecular functions. We found that Pol II subunits contribute differently to Pol II cellular localization and transcription process and preferentially regulate RNA processing (such as RNA splicing and 3’ end maturation). Genes sensitive to the depletion of different Pol II subunits tend to be involved in diverse biological functions and show different RNA half-lives. Sequences, associated protein factors, and RNA structures are correlated with Pol II subunit-mediated differential gene expression. These findings collectively suggest that the heterogeneity of Pol II and different genes appear to depend on some of the subunits.
HostingRepository	iProX
AnnounceDate	2022-08-09
AnnouncementXML	Submission_2023-12-21_20:44:28.277.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiong Ji
SpeciesList	scientific name: Mus ; NCBI TaxID: 10088;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-08 00:08:52	ID requested
1	2022-08-08 00:09:13	announced
2	2022-08-08 19:59:14	announced	2022-08-09: Update information.
⏵ 3	2023-12-21 20:44:29	announced	2022-08-09: Update information. 2023-12-22: Update publication information.

Li Y, Huang J, Zhu J, Bao L, Wang H, Jiang Y, Tian K, Wang R, Zheng H, Duan W, Lai W, Yi X, Zhu Y, Guo T, Ji X, Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity. Mol Cell, 82(20):3943-3959.e11(2022) [pubmed]

submitter keyword: Transcription, Pol II subunits, ChIP-MS

Xiong Ji
contact affiliation	Peking University
contact email	xiongji@pku.edu.cn
lab head
Xiong Ji
contact affiliation	Peking University
contact email	xiongji@pku.edu.cn
dataset submitter

iProX dataset URI