PXD035849
PXD035849 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS identification of proteins that were bound to recombinant His-tagged isoforms of RPL22L1 |
Description | Glioblastoma (GBM) is characterized by an exceptionally high intratumoral heterogeneity. However, the molecular mechanisms underlying the origin of different GBM cell populations remain unclear. Here we found that the composition of ribosomes of GBM cells in the tumor core and edge differ due to alternative RNA splicing. The acidic pH in the core switches pre-mRNA splicing of the ribosomal gene RPL22L1 toward the RPL22L1b isoform. To elucidate the functions of RPL22L1 isoforms we identified proteins that interact with RPL22L1a and RPL22L1b. First, each isoform was expressed in E. coli and immobilized on magnetic beads. The beads were incubated with GBM cell lysates and proteins bound to the beads were identified using LC-MS/MS. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:06:52.082.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD035849 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Georgij Arapidi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-08-07 03:07:39 | ID requested | |
1 | 2023-10-24 10:55:18 | announced | |
⏵ 2 | 2023-11-14 09:06:54 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD035849; |
Larionova TD, Bastola S, Aksinina TE, Anufrieva KS, Wang J, Shender VO, Andreev DE, Kovalenko TF, Arapidi GP, Shnaider PV, Kazakova AN, Latyshev YA, Tatarskiy VV, Shtil AA, Moreau P, Giraud F, Li C, Wang Y, Rubtsova MP, Dontsova OA, Condro M, Ellingson BM, Shakhparonov MI, Kornblum HI, Nakano I, Pavlyukov MS, Alternative RNA splicing modulates ribosomal composition and determines the spatial phenotype of glioblastoma cells. Nat Cell Biol, 24(10):1541-1557(2022) [pubmed] |
Keyword List
submitter keyword: Glioblastoma, ribosome, RNA splicing, RPL22L1, intratumoral heterogeneity |
Contact List
Georgij Arapidi | |
---|---|
contact affiliation | Head of System Biology Lab. at Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Russia |
contact email | arapidi@gmail.com |
lab head | |
Georgij Arapidi | |
contact affiliation | Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
contact email | arapidi@gmail.com |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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