PXD035848

PXD035848 is an original dataset announced via ProteomeXchange.

Title	Cell-type resolution proteomics for functional annotation of the grain filling process in wheat endosperm
Description	Grain development is a key life cycle stage of many plants. The development of seeds is the basis of agriculture and the primary source of calories consumed by humans. Here, we employ laser micro dissection (LMD) combined with shotgun proteomics to generate a cell-type proteome atlas of developing wheat endosperm at the early and late grain filling stages. We identified 1803 proteins from four different cell layers (aleurone (AL), sub-aleurone (SA), starchy endosperm (SE), and endosperm transfer cells (ETCs)) of developing endosperm at 15 Days after anthesis (DAA) and 26 DAA. Sixty-seven differentially expressed proteins in the aleurone, 31 in the sub-aleurone, 27 in the starchy endosperm, and 50 in the endosperm transfer cells were detected between these two-time points. The results revealed highly distinguishable proteome dynamics in the different cell layers of endosperm over the time course. We observed high general metabolic activity of the grain with regard to carbohydrate metabolism, defence against oxidative stress, and signalling in the different cell layers during the grain filling process. Cell-specific identification of SUT and GLUT transporters suggest a grain filling model via nucellar projections and endosperm transfer cells (ETCs) initiating starch biosynthesis in the starchy endosperm (SE). The identification and regulation dynamics of proteins in the different cell layers demonstrate a functional switch of the proteome from the early to the late grain filling stage. Based on these data, we proposed a model for sugar loading and starch biosynthesis in wheat developing endosperm, including an abundance switch of cell-type-specific key proteins.
HostingRepository	PRIDE
AnnounceDate	2024-12-11
AnnouncementXML	Submission_2024-12-11_14:56:24.984.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Palak Chaturvedi
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-07 03:07:17	ID requested
⏵ 1	2024-12-11 14:56:25	announced

10.1111/PBI.14203;

submitter keyword: proteomics, laser micro dissection (LMD), starchy endosperm, transfer cells,Wheat, sub aleurone, aleurone

Palak Chaturvedi
contact affiliation	Molecular Systems Biology (MOSYS), Department of Functional and Evolutionary Ecology, University of Vienna, Austria
contact email	palak.chaturvedi@univie.ac.at
lab head
Palak Chaturvedi
contact affiliation	University of Vienna
contact email	palak.chaturvedi@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD035848

PRIDE project URI

• PRIDE
  1. PXD035848
     1. Label: PRIDE project
     2. Name: Cell-type resolution proteomics for functional annotation of the grain filling process in wheat endosperm