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PXD035842

PXD035842 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic Evaluation of the Influence of Shear Stress and BRD4-Inhibition on Human Umbilical Vein Endothelial Cells
DescriptionAtherosclerosis is an important pathological factor in the development of cardiovascular diseases. In addition to increased plasma lipid concentrations, irregular/oscillatory shear stress and inflammatory processes trigger pathophysiological changes. Inhibitors of the transcription modulatory bromo- and extra-terminal domain (BET) protein family (BETi) could offer a possible therapeutic approach due to their anti-inflammatory properties. In this study, the influence of laminar shear stress, inflammation and BETi on human endothelial cells in an atherosclerosis in vitro model was investigated using global protein expression profiling. For this purpose, human umbilical cord derived vascular endothelial cells (HUVEC) were treated with TNFα to mimic the inflammatory condition and were exposed to 24h laminar shear stress in the presence or absence of a BRD4 inhibitor, JQ1. Data-independent acquisition mass spectrometry (DIA-MS) alloqed us to quantify 3316 proteins for further statistical analysis. Differentially regulated proteins indicate a clear influence of inflammation and shear stress on human endothelial cells. Overall, application of JQ1 is led to significant changes in the proteome, including a strong anti-inflammatory response as well as a potentially negative impact on atherosclerosis formation. To our knowledge, this is the first proteomics study on HUVEC which investigates the influence of shear stress and BET inhibition in TNFα inflammatory endothelial cell culture model.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:29:09.654.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristof Lenz
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro 2
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-07 03:05:06ID requested
12022-10-30 11:56:12announced
22023-11-14 08:29:11announced2023-11-14: Updated project metadata.
Publication List
Jarausch J, Neuenroth L, Andag R, Leha A, Fischer A, Asif AR, Lenz C, Eidizadeh A, Influence of Shear Stress, Inflammation and BRD4 Inhibition on Human Endothelial Cells: A Holistic Proteomic Approach. Cells, 11(19):(2022) [pubmed]
Keyword List
submitter keyword: Shear Stress, BRD4, Atherosclerosis, DIA-MS, JQ1, Endothelial, BET-Inhibitor,HUVEC, Proteomics, LC-MS/MS
Contact List
Christof Lenz
contact affiliationUniversity Medical Center, Department of Clinical Chemistry, Core Facility Proteomics
contact emailchristof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliationMax Planck Institute for Biophysical Chemistry
contact emailchristof.lenz@mpibpc.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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