PXD035842 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic Evaluation of the Influence of Shear Stress and BRD4-Inhibition on Human Umbilical Vein Endothelial Cells |
Description | Atherosclerosis is an important pathological factor in the development of cardiovascular diseases. In addition to increased plasma lipid concentrations, irregular/oscillatory shear stress and inflammatory processes trigger pathophysiological changes. Inhibitors of the transcription modulatory bromo- and extra-terminal domain (BET) protein family (BETi) could offer a possible therapeutic approach due to their anti-inflammatory properties. In this study, the influence of laminar shear stress, inflammation and BETi on human endothelial cells in an atherosclerosis in vitro model was investigated using global protein expression profiling. For this purpose, human umbilical cord derived vascular endothelial cells (HUVEC) were treated with TNFα to mimic the inflammatory condition and were exposed to 24h laminar shear stress in the presence or absence of a BRD4 inhibitor, JQ1. Data-independent acquisition mass spectrometry (DIA-MS) alloqed us to quantify 3316 proteins for further statistical analysis. Differentially regulated proteins indicate a clear influence of inflammation and shear stress on human endothelial cells. Overall, application of JQ1 is led to significant changes in the proteome, including a strong anti-inflammatory response as well as a potentially negative impact on atherosclerosis formation. To our knowledge, this is the first proteomics study on HUVEC which investigates the influence of shear stress and BET inhibition in TNFα inflammatory endothelial cell culture model. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:29:09.654.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christof Lenz |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro 2 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-07 03:05:06 | ID requested | |
1 | 2022-10-30 11:56:12 | announced | |
⏵ 2 | 2023-11-14 08:29:11 | announced | 2023-11-14: Updated project metadata. |
Publication List
Jarausch J, Neuenroth L, Andag R, Leha A, Fischer A, Asif AR, Lenz C, Eidizadeh A, Influence of Shear Stress, Inflammation and BRD4 Inhibition on Human Endothelial Cells: A Holistic Proteomic Approach. Cells, 11(19):(2022) [pubmed] |
Keyword List
submitter keyword: Shear Stress, BRD4, Atherosclerosis, DIA-MS, JQ1, Endothelial, BET-Inhibitor,HUVEC, Proteomics, LC-MS/MS |
Contact List
Christof Lenz |
contact affiliation | University Medical Center, Department of Clinical Chemistry, Core Facility Proteomics |
contact email | christof.lenz@med.uni-goettingen.de |
lab head | |
Christof Lenz |
contact affiliation | Max Planck Institute for Biophysical Chemistry |
contact email | christof.lenz@mpibpc.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035842 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035842
- Label: PRIDE project
- Name: Proteomic Evaluation of the Influence of Shear Stress and BRD4-Inhibition on Human Umbilical Vein Endothelial Cells