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PXD035836

PXD035836 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome of hemodialysis filter membranes
DescriptionUsed hemodialysis filter membranes (HD filters) are discarded as waste products but represent a reservoir of valuable biological information. Numerous serum proteins are known to bind to HD filters, but whether this process selectively affects individual protein classes has not been adequately elucidated. Modern proteomics analyses offer the possibility to identify and quantify this therapy-specific subproteome and, in combination with bioinformatics methods, allow the analysis of the associated metabolic pathways. The description of the proteins at a HD filter membrane could provide insights into important modulators of the immune system or pathophysiological processes at the patient level. The aim of this project is to characterize the extracorporeal proteome of HD patients. HD filters were continuously rinsed with physiological saline immediately after the end of the HD session to remove most of the remaining blood from the capillaries. Then, a chaotropic buffer was circulated in the system for 1h by peristaltic pump to elute adsorbed proteins. Enzymatically digested proteins were desalted and purified, and separated in technical duplicate by liquid chromatography and analyzed by Orbitrap mass spectrometer, and identified bioinformatically.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:38:48.117.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKlaus Kratochwill
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Orbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-07 03:02:23ID requested
12023-03-10 23:51:27announced
22023-11-14 08:38:50announced2023-11-14: Updated project metadata.
Publication List
Daniel-Fischer L, Sobieszek IJ, Wagner A, Sacnun JM, Watschinger B, Aufricht C, Kratochwill K, Herzog R, In-Depth Analysis of the Extracorporeal Proteome Adsorbed to Dialysis Membranes during Hemodialysis. Membranes (Basel), 12(11):(2022) [pubmed]
Keyword List
submitter keyword: hemodialysis
Contact List
Dr Klaus Kratochwill
contact affiliationMedical University of Vienna
contact emailklaus.kratochwill@meduniwien.ac.at
lab head
Klaus Kratochwill
contact affiliationMedical University of Vienna
contact emailklaus.kratochwill@meduniwien.ac.at
dataset submitter
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Dataset FTP location
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