PXD035836 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome of hemodialysis filter membranes |
Description | Used hemodialysis filter membranes (HD filters) are discarded as waste products but represent a reservoir of valuable biological information. Numerous serum proteins are known to bind to HD filters, but whether this process selectively affects individual protein classes has not been adequately elucidated. Modern proteomics analyses offer the possibility to identify and quantify this therapy-specific subproteome and, in combination with bioinformatics methods, allow the analysis of the associated metabolic pathways. The description of the proteins at a HD filter membrane could provide insights into important modulators of the immune system or pathophysiological processes at the patient level. The aim of this project is to characterize the extracorporeal proteome of HD patients. HD filters were continuously rinsed with physiological saline immediately after the end of the HD session to remove most of the remaining blood from the capillaries. Then, a chaotropic buffer was circulated in the system for 1h by peristaltic pump to elute adsorbed proteins. Enzymatically digested proteins were desalted and purified, and separated in technical duplicate by liquid chromatography and analyzed by Orbitrap mass spectrometer, and identified bioinformatically. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:38:48.117.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Klaus Kratochwill |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-07 03:02:23 | ID requested | |
1 | 2023-03-10 23:51:27 | announced | |
⏵ 2 | 2023-11-14 08:38:50 | announced | 2023-11-14: Updated project metadata. |
Publication List
Daniel-Fischer L, Sobieszek IJ, Wagner A, Sacnun JM, Watschinger B, Aufricht C, Kratochwill K, Herzog R, In-Depth Analysis of the Extracorporeal Proteome Adsorbed to Dialysis Membranes during Hemodialysis. Membranes (Basel), 12(11):(2022) [pubmed] |
Keyword List
submitter keyword: hemodialysis |
Contact List
Dr Klaus Kratochwill |
contact affiliation | Medical University of Vienna |
contact email | klaus.kratochwill@meduniwien.ac.at |
lab head | |
Klaus Kratochwill |
contact affiliation | Medical University of Vienna |
contact email | klaus.kratochwill@meduniwien.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035836 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035836
- Label: PRIDE project
- Name: Proteome of hemodialysis filter membranes