PXD035819

PXD035819 is an original dataset announced via ProteomeXchange.

Title	Phospho-regulation of Cdc48/p97 cofactor protein Shp1/p47 in budding yeast
Description	Shp1/p47 is a cofactor of the Cdc48/p97 complex involved in the energy-dependent segregation of intracellular aggregates and multiprotein complexes. We identified two serine residues (S108 and S315) in the S. Cerevisiae protein Shp1 which increase in phosphorylation upon various cell stresses such as heat shock, TORC1 inhibition, ER stress and oxidative stress. To identify binding partners of phosphorylated vs non-phosphorylated Shp1, we generated endogenous C-terminal 5xFLAG-tagged Shp1 yeast strains either harbouring either WT shp1 or CRISPR-modified S108A + S315A (2SA) shp1. These were cultured with 200 nM rapamycin treatment for 2 h to induce phosphorylation, prior to lysis, FLAG immunoprecipitation, peptide digestion and processing for LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:07:34.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frederic Lamoliatte
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-04 08:19:53	ID requested
1	2023-01-30 04:07:30	announced
⏵ 2	2023-11-14 08:07:35	announced	2023-11-14: Updated project metadata.

Agrotis A, Lamoliatte F, Williams TD, Black A, Horberry R, Rousseau A, Multiple phosphorylation of the Cdc48/p97 cofactor protein Shp1/p47 occurs upon cell stress in budding yeast. Life Sci Alliance, 6(4):(2023) [pubmed]

submitter keyword: Ubx domain, stress, phosphorylation, TORC1, protein degradation, Ubx1, proteostasis

Adrien Rousseau
contact affiliation	Medical Research Council Protein Phosphorylation and Ubiquitylation Unit School of Life Sciences, University of Dundee, Dundee, Scotland
contact email	a.rousseau@dundee.ac.uk
lab head
Frederic Lamoliatte
contact affiliation	University of Dundee
contact email	flamoliatte001@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD035819

PRIDE project URI

• PRIDE
  1. PXD035819
     1. Label: PRIDE project
     2. Name: Phospho-regulation of Cdc48/p97 cofactor protein Shp1/p47 in budding yeast