PXD035813

PXD035813 is an original dataset announced via ProteomeXchange.

Title	A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of mitochondria-lipid droplet contact proteomes
Description	Accumulating studies have shown that mitochondria-lipid droplet (LD) interaction is crucial for maintaining the homeostasis of lipid metabolism in the liver. However, the proteins involved in mitochondria-LD interaction and the functioning mechanism remain largely elusive. Here, we applied a systematic approach to characterize the proteome of mitochondria-LD contacts under normal or starvation condition by combining the bimolecular fluorescence complementary assay and a proximity labeling strategy. We first established a reporter of mitochondria-LD contacts in HepG2 cells and demonstrated that mitochondria-LD contacts are pretty sensitive to nutritional stresses. Furthermore, we uncovered 60 proteins are up-or down-regulated at contact sites upon starvation. Finally, we validated that SQLE translocates from endoplasmic reticulum to LDs and accumulates at mitochondria-LD contact sites upon starvation so as to utilize local ATP for cholesterol synthesis. This work also provides a versatile tool to profile the proteomes of any other inter-organelle membrane contacts in living cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:51.498.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maoge Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-04 08:01:12	ID requested
1	2023-10-24 10:54:45	announced
⏵ 2	2023-11-14 09:06:52	announced	2023-11-14: Updated project metadata.

Zhou M, Kong B, Zhang X, Xiao K, Lu J, Li W, Li M, Li Z, Ji W, Hou J, Xu T, A proximity labeling strategy enables proteomic analysis of inter-organelle membrane contacts. iScience, 26(7):107159(2023) [pubmed]

submitter keyword: cholesterol, BiFC, comparative proteomics, proximity labeling,Mitochondria-lipid droplet contact

Tao Xu
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
contact email	xutao@ibp.ac.cn
lab head
Maoge Zhou
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	fyps200799@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD035813

PRIDE project URI

• PRIDE
  1. PXD035813
     1. Label: PRIDE project
     2. Name: A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of mitochondria-lipid droplet contact proteomes