PXD035801

PXD035801 is an original dataset announced via ProteomeXchange.

Title	A Proximity Labeling Strategy Enables Spatial Proteomic Analysis of Inter-organelle Membrane Contacts in Living Cells
Description	Inter-organelle membrane contacts especially mitochondria-endoplasmic reticulum contacts (MERC) conduct important biological functions including exchange of lipids and ions, and modulation of membrane dynamics. Alterations of inter-organelle membrane contacts have been implicated with the pathogenesis of diseases, such as neurodegenerative diseases, cancers and type 2 diabetes. However, the protein compositions of inter-organelle contacts remain largely unknown as no biomarker has been discovered and it’s difficult to purify intact inter-organelle contact proteins. Here, we applied a systematic approach to probe the spatial proteome of MERC in living cells by combining the bimolecular fluorescence complementation assay and a proximity labeling strategy based on APEX2. As a result, we discovered 403 highly confident MERC proteins including many well-known MERC proteins and a variety of novel protein species as well. We further validated that WFS1, BAG2, SPTLC1 and GLUD1 are enriched at MERC with high resolution fluorescent imaging. Our study provides a powerful tool to characterize the spatial proteomes of inter-organelle membrane contacts in living cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:08:35.305.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maoge Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-04 07:55:08	ID requested
1	2023-10-24 11:04:50	announced
2	2023-11-14 09:07:11	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:08:36	announced	2024-10-22: Updated project metadata.

10.1016/j.isci.2023.107159;

Zhou M, Kong B, Zhang X, Xiao K, Lu J, Li W, Li M, Li Z, Ji W, Hou J, Xu T, A proximity labeling strategy enables proteomic analysis of inter-organelle membrane contacts. iScience, 26(7):107159(2023) [pubmed]

submitter keyword: APEX, BiFC,MERC, proteome

Tao Xu
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
contact email	xutao@ibp.ac.cn
lab head
Maoge Zhou
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	fyps200799@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD035801

PRIDE project URI

• PRIDE
  1. PXD035801
     1. Label: PRIDE project
     2. Name: A Proximity Labeling Strategy Enables Spatial Proteomic Analysis of Inter-organelle Membrane Contacts in Living Cells