PXD035784

PXD035784 is an original dataset announced via ProteomeXchange.

Title	C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse
Description	Extramedullary infiltration (EMI) is a concomitant manifestation indicating poor prognosis of acute myeloid leukemia (AML), yet the underlying mechanism remains poorly understood and therapeutic options are limited. Here, we employed single-cell RNA sequencing on bone marrow (BM) and EMI samples from an AML patient presenting pervasive leukemia cutis. A complement C1Q + macrophage-like leukemia subset which is enriched within cutis and existed in BM prior to EMI manifestations was identified and further verified in multiple AML patients. RNA-sequencing and quantitative proteomics profiling revealed adverse prognosis of patients bearing C1Q + population. C1Q expression endowed leukemia cells with tissue-infiltration ability which could establish prominent cutaneous or gastrointestinal EMI nodules in patient-derived xenograft (PDX) and CDX models. Fibroblasts attracted migration of the C1Q + leukemia cells through surface C1Q-gC1QR recognition and subsequent stimulation of MAFB-TGF-β signaling. Thus, C1Q orchestrates cancer infiltration pathways through communicating with fibroblasts and represents a compelling therapeutic target for EMI.
HostingRepository	iProX
AnnounceDate	2022-08-03
AnnouncementXML	Submission_2023-12-06_20:14:52.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li Xia
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 19:26:48	ID requested
⏵ 1	2023-12-06 20:14:53	announced

Yang LX, Zhang CT, Yang MY, Zhang XH, Liu HC, Luo CH, Jiang Y, Wang ZM, Yang ZY, Shi ZP, Yang YC, Wei RQ, Zhou L, Mi J, Zhou AW, Yao ZR, Xia L, Yan JS, Lu Y, C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse. Blood, 141(7):766-786(2023) [pubmed]

submitter keyword: C1Q, CD34+, AML, DIA, Proteome

Ying Lu
contact affiliation	Xinhua Hospital, Shanghai Jiao Tong University School of Medicine
contact email	stove@shsmu.edu.cn
lab head
Li Xia
contact affiliation	Shanghai Jiao Tong University School of Medicine
contact email	lixia@shsmu.edu.cn
dataset submitter

iProX dataset URI