PXD035779

PXD035779 is an original dataset announced via ProteomeXchange.

Title	The PqsE Protein Interaction Network in Pseudomonas aeruginosa
Description	The PqsE enzyme plays a vital role in quorum sensing and virulence in Pseudomonas aeruginosa, yet its enzymatic function is unknown. Here, we identify the protein interaction network of PqsE as well as that of a catalytically dead variant, PqsE(D73A) in P. aeruginosa PA14. Our analyses identify proteins that interact with PqsE that are independent of and that depend on PqsE catalytic function. One such catalysis-independent interaction is with the quorum-sensing regulator, RhlR, consistent with our previous work. We also characterize the PqsE interaction network in a delta rhlR P. aeruginosa PA14 strain and identify additional proteins as PqsE-interactors.
HostingRepository	PRIDE
AnnounceDate	2022-09-06
AnnouncementXML	Submission_2022-09-06_08:56:23.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Todd Greco
SpeciesList	scientific name: Pseudomonas sp.; NCBI TaxID: 306;
ModificationList	phosphorylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 11:07:58	ID requested
⏵ 1	2022-09-06 08:56:23	announced

Dataset with its publication pending

submitter keyword: Bacteria, Quorum sensing, Q-Exactive HF, IP-MS

Ileana M Cristea
contact affiliation	Molecular Biology, Princeton University, USA
contact email	icristea@princeton.edu
lab head
Todd Greco
contact affiliation	Molecular Biology, Princeton University
contact email	tgreco@princeton.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035779
     1. Label: PRIDE project
     2. Name: The PqsE Protein Interaction Network in Pseudomonas aeruginosa