PXD035778

PXD035778 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM
Description	The spike protein of the porcine epidemic diarrhea virus from various strains was generated from different mammalian expression systems. The integration of cryo-EM and MS delineated the glycosylation patterns on Spike proteins and also helped understand how individual glycans contribute to biological functions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:23:31.834.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035778
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yu-Chun Chien
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 11:07:42	ID requested
1	2022-10-14 21:50:28	announced
⏵ 2	2023-11-14 07:23:32	announced	2023-11-14: Updated project metadata.

Publication List

10.6019/PXD035778;
Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD, In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM. Nat Commun, 13(1):4877(2022) [pubmed]

10.6019/PXD035778;

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD, In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM. Nat Commun, 13(1):4877(2022) [pubmed]

Keyword List

submitter keyword: PEDV, spike protein, alphacoronavirus

submitter keyword: PEDV, spike protein, alphacoronavirus

Contact List

Kay-Hooi Khoo
contact affiliation	Institute of Biological Chemistry, Academia Sinica
contact email	kkhoo@gate.sinica.edu.tw
lab head
Yu-Chun Chien
contact affiliation	Academia sinica
contact email	cgubs20@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035778
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035778

PRIDE project URI

Repository Record List

[ + ]