PXD035751

PXD035751 is an original dataset announced via ProteomeXchange.

Title	Quantitative Phosphoproteomics Analyses Reveal the Regulatiory Mechanisms Related to Frozen-thawed Sperm Capacitation and Acrosome Reaction in Yak (Bos grunniens)
Description	Mammalian spermatozoa are not utterly mature after ejaculation and must undergo additional functional and structural changes within female reproductive tracts to achieve subsequent fertilization, including both capacitation and acrosome reaction (AR), which are dominated by post-translational modifications (PTMs), especially phosphorylation. However, the mechanisms of protein phosphorylation during frozen-thawed sperm capacitation and AR has not yet been well studied. We employed phosphoproteomic approach based on TMT labeling combined with LC-MS/MS strategy to analyze frozen-thawed sperm in Ashidan yak under three sequential conditions (density gradient centrifugation-based purification, incubation in the capacitation medium and induction of AR processes by the calcium ionophore A23187 treatment).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:20.612.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhang renzheng
SpeciesList	scientific name: Bos mutus grunniens (Wild yak) (Bos grunniens); NCBI TaxID: 30521;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 10:33:55	ID requested
1	2022-09-25 18:41:53	announced
⏵ 2	2023-11-14 08:57:21	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: yak sperm phosphoproteomics TMT LC-MS/MS

Zhang Renzheng
contact affiliation	Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China
contact email	zhangrenzheng51@163.com
lab head
zhang renzheng
contact affiliation	PTM Biolabs Inc.
contact email	zhangrenzheng51@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD035751

PRIDE project URI

• PRIDE
  1. PXD035751
     1. Label: PRIDE project
     2. Name: Quantitative Phosphoproteomics Analyses Reveal the Regulatiory Mechanisms Related to Frozen-thawed Sperm Capacitation and Acrosome Reaction in Yak (Bos grunniens)