PXD035741 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Detailing the Structural and Dynamic Interactions Between WDR44 and Rab11 |
Description | DR44 is a Rab11 effector protein that is thought to regulate ciliogenesis by competing with pro-ciliogenesis factors for Rab11 binding. The structure of WDR44 and the molecular mechanism of its interaction with Rab11 is unknown. To explore the interactions between these two proteins multiple WDR44 constructs were designed and pulled down with Rab11. Using the results of the pulldown assay as a guide we used protein complex prediction software, and hydrogen deuterium exchange mass spectrometry (HDX-MS) to identify the binding interface between WDR44 and Rab11. Mutagenesis was used to make specific mutations in the putative Rab11 binding region of WDR44. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:42:53.051.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | John Burke |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | impact HD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-02 10:20:28 | ID requested | |
1 | 2023-03-11 05:14:56 | announced | |
⏵ 2 | 2023-11-14 08:42:53 | announced | 2023-11-14: Updated project metadata. |
Publication List
Thibodeau MC, Harris NJ, Jenkins ML, Parson MAH, Evans JT, Scott MK, Shaw AL, Pokorn, ý D, Leonard TA, Burke JE, Molecular basis for the recruitment of the Rab effector protein WDR44 by the GTPase Rab11. J Biol Chem, 299(1):102764(2023) [pubmed] |
Keyword List
submitter keyword: HDX-MS,WDR44, Rab11 |
Contact List
Dr. John E. Burke |
contact affiliation | University of Victoria |
contact email | jeburke@uvic.ca |
lab head | |
John Burke |
contact affiliation | University of Victoria |
contact email | jeburke@uvic.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035741
- Label: PRIDE project
- Name: Detailing the Structural and Dynamic Interactions Between WDR44 and Rab11