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PXD035741

PXD035741 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDetailing the Structural and Dynamic Interactions Between WDR44 and Rab11
DescriptionDR44 is a Rab11 effector protein that is thought to regulate ciliogenesis by competing with pro-ciliogenesis factors for Rab11 binding. The structure of WDR44 and the molecular mechanism of its interaction with Rab11 is unknown. To explore the interactions between these two proteins multiple WDR44 constructs were designed and pulled down with Rab11. Using the results of the pulldown assay as a guide we used protein complex prediction software, and hydrogen deuterium exchange mass spectrometry (HDX-MS) to identify the binding interface between WDR44 and Rab11. Mutagenesis was used to make specific mutations in the putative Rab11 binding region of WDR44.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:42:53.051.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJohn Burke
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
Instrumentimpact HD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-02 10:20:28ID requested
12023-03-11 05:14:56announced
22023-11-14 08:42:53announced2023-11-14: Updated project metadata.
Publication List
Thibodeau MC, Harris NJ, Jenkins ML, Parson MAH, Evans JT, Scott MK, Shaw AL, Pokorn, ý D, Leonard TA, Burke JE, Molecular basis for the recruitment of the Rab effector protein WDR44 by the GTPase Rab11. J Biol Chem, 299(1):102764(2023) [pubmed]
Keyword List
submitter keyword: HDX-MS,WDR44, Rab11
Contact List
Dr. John E. Burke
contact affiliationUniversity of Victoria
contact emailjeburke@uvic.ca
lab head
John Burke
contact affiliationUniversity of Victoria
contact emailjeburke@uvic.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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