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PXD035738

PXD035738 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHILIC enrichment of Leishmania major MHOM
DescriptionThe trypanosomatid protozoan parasite Leishmania has a significant impact on human health globally. Understanding the pathways associated with virulence within this significant pathogen is critical for identifying novel vaccination and chemotherapy targets. Within this study we leverage an ultradeep proteomic approach to improve our understanding of two virulence associated genes in Leishmania; the Golgi Mannose/Arabinopyranose/Fucose nucleotide-sugar transporter LPG2, and the mitochondrial fucosyltransferase FUT1. Using deep peptide fractionation followed by complementary fragmentation approaches with higher energy collisional dissociation (HCD) and Electron-transfer dissociation (ETD) allowed the identification of over 6500 proteins, nearly doubling the experimentally observed Leishmania major proteome. This deep proteomic analysis revealed significant quantitative differences in both lpg2- and fut1s mutants with FUT1-dependent changes linked to marked alterations within mitochondrial associated proteins while LPG2-dependent changes impacted multiple aspects of the secretory pathway. While FUT1 has been shown to fucosylate peptides in vitro, no evidence for protein fucosylation was identified within our ultradeep analysis nor did we observe fucosylated glycans within Leishmania glycopeptides isolated using HILIC enrichment. Combined this work provides a critical proteomic resource for the community on the observable Leishmania proteome as well as highlights phenotypic changes associated with LPG2/FUT1 which may guide the development of future therapeutics.
HostingRepositoryPRIDE
AnnounceDate2022-10-13
AnnouncementXMLSubmission_2022-10-13_02:52:24.446.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNichollasScott
SpeciesList scientific name: Leishmania major species complex; NCBI TaxID: 38581;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-02 10:19:27ID requested
12022-10-13 02:52:24announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Leishmania major MHOM,Glycopeptides
Contact List
Stephen M.Beverley
contact affiliationDept. of Molecular Microbiology, Washington University School of Medicine, St. Louis. MO 63110 USA; 314-747-2630
contact emailstephen.beverley@wustl.edu
lab head
NichollasScott
contact affiliationUniversity of Melbourne
contact emailnichollas.scott@unimelb.edu.au
dataset submitter
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Dataset FTP location
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