PXD035738 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | HILIC enrichment of Leishmania major MHOM |
Description | The trypanosomatid protozoan parasite Leishmania has a significant impact on human health globally. Understanding the pathways associated with virulence within this significant pathogen is critical for identifying novel vaccination and chemotherapy targets. Within this study we leverage an ultradeep proteomic approach to improve our understanding of two virulence associated genes in Leishmania; the Golgi Mannose/Arabinopyranose/Fucose nucleotide-sugar transporter LPG2, and the mitochondrial fucosyltransferase FUT1. Using deep peptide fractionation followed by complementary fragmentation approaches with higher energy collisional dissociation (HCD) and Electron-transfer dissociation (ETD) allowed the identification of over 6500 proteins, nearly doubling the experimentally observed Leishmania major proteome. This deep proteomic analysis revealed significant quantitative differences in both lpg2- and fut1s mutants with FUT1-dependent changes linked to marked alterations within mitochondrial associated proteins while LPG2-dependent changes impacted multiple aspects of the secretory pathway. While FUT1 has been shown to fucosylate peptides in vitro, no evidence for protein fucosylation was identified within our ultradeep analysis nor did we observe fucosylated glycans within Leishmania glycopeptides isolated using HILIC enrichment. Combined this work provides a critical proteomic resource for the community on the observable Leishmania proteome as well as highlights phenotypic changes associated with LPG2/FUT1 which may guide the development of future therapeutics. |
HostingRepository | PRIDE |
AnnounceDate | 2022-10-13 |
AnnouncementXML | Submission_2022-10-13_02:52:24.446.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | NichollasScott |
SpeciesList | scientific name: Leishmania major species complex; NCBI TaxID: 38581; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-02 10:19:27 | ID requested | |
⏵ 1 | 2022-10-13 02:52:24 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Leishmania major MHOM,Glycopeptides |
Contact List
Stephen M.Beverley |
contact affiliation | Dept. of Molecular Microbiology, Washington University School of Medicine, St. Louis. MO 63110 USA; 314-747-2630 |
contact email | stephen.beverley@wustl.edu |
lab head | |
NichollasScott |
contact affiliation | University of Melbourne |
contact email | nichollas.scott@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035738
- Label: PRIDE project
- Name: HILIC enrichment of Leishmania major MHOM