PXD035737

PXD035737 is an original dataset announced via ProteomeXchange.

Title	Crosstalk of Protein Expression and Lysine Acetylation in Response to TMV Infection in Nicotiana benthamiana
Description	Kac, a reversible PTM, plays essential roles in various biological processes, including those involving metabolic pathways, pathogen resistance and transcription, in both prokaryotes and eukaryotes. TMV, the major factor that causes poor quality of Solanaceae crops worldwide, directly alters many metabolic processes in tobacco. However, the extent and function of Kac during TMV infection have not been determined. Here, using LC−MS/MS in conjunction with highly sensitive immune-affinity purification, we comprehensively analyzed the changes in the proteome and acetylome of TMV-infected tobacco (Nicotiana benthamiana) seedlings. In total, 2082 lysine-acetylated sites on 1319 proteins differentially expressed in response to TMV infection were identified. Extensive bioinformatic studies disclosed changes in acetylation of proteins engaged in cellular metabolism and biological processes. The vital influence of Kac in fatty acid degradation and alpha-linolenic acid metabolism was also revealed in TMV-infected seedlings. This study first revealed Kac information in N. benthamiana under TMV infection and expands upon the existing landscape of acetylation in pathogen infection.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:30:22.908.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xianliang Ma
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 10:19:05	ID requested
1	2022-10-14 20:28:11	announced
⏵ 2	2023-11-14 08:30:23	announced	2023-11-14: Updated project metadata.

Song L, Zhan H, Wang Y, Lin Z, Li B, Shen L, Jiao Y, Li Y, Wang F, Yang J, . ACS Omega, 7(36):32496-32511(2022) [pubmed]

submitter keyword: lysine acetylation,TMV, Nicotiana benthamiana, proteome

Jinguang Yang
contact affiliation	Tobacco Research Institute of CAAS
contact email	yangjinguang@caas.cn
lab head
Xianliang Ma
contact affiliation	Micrometer Biotech
contact email	maxianliangyxz@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035737
     1. Label: PRIDE project
     2. Name: Crosstalk of Protein Expression and Lysine Acetylation in Response to TMV Infection in Nicotiana benthamiana