PXD035726

PXD035726 is an original dataset announced via ProteomeXchange.

Title	Human serum samples - Acute and chronic blood serum proteome changes in patients with methanol poisoning
Description	We carried out a prospective, longitudinal, single-center, observational cohort study of patients with confirmed acute methanol poisoning that were treated in hospitals during a mass methanol poisoning outbreak in the Czech Republic in 2012. Venous blood for proteomic analysis was obtained from 24 patients with confirmed acute methanol poisoning upon admission to the hospital (group M (“Methanol”)) with heparin administration for hemodialysis and ethanol or fomepizole administration as the antidote to block ADH. In the follow-up group of survivors of methanol poisoning (group S (“Survivors”)), venous blood samples for proteomic analysis were obtained from 46 patients during the examination, which took place 4 years after discharge from the hospital. For the control group not exposed to methanol, 24 healthy subjects were recruited (group C, “Controls”). Blood samples were spun, the serum was separated, and the samples were frozen to −80 °C until the analyses. Blood serum samples were depleted of most abundant serum proteins using Agilent MARS 14 column, samples fractionated and fractions containing proteins of interest precipitated. Samples were analyzed using LC-MS/MS Thermo Orbitrap Fusion (UHPLC-ESI-Q-OT-qIT) and identified proteins with differential expression.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:35:33.712.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Cejnar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 02:19:51	ID requested
1	2022-12-12 03:06:45	announced
⏵ 2	2023-11-14 07:35:35	announced	2023-11-14: Updated project metadata.

Cejnar P, Smirnova TA, Kuckova S, Prochazka A, Zak I, Harant K, Zakharov S, Acute and chronic blood serum proteome changes in patients with methanol poisoning. Sci Rep, 12(1):21379(2022) [pubmed]

submitter keyword: LC-MS/MS, Human, acute poisoning, serum, methanol

Pavel Cejnar
contact affiliation	Department of Computing and Control Engineering, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha 6, Czech Republic
contact email	pavel.cejnar@vscht.cz
lab head
Pavel Cejnar
contact affiliation	Department of Computing and Control Engineering, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha 6, Czech Republic
contact email	pavel.cejnar@vscht.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD035726

PRIDE project URI

• PRIDE
  1. PXD035726
     1. Label: PRIDE project
     2. Name: Human serum samples - Acute and chronic blood serum proteome changes in patients with methanol poisoning