PXD035724

PXD035724 is an original dataset announced via ProteomeXchange.

Title	Proteomic screening for potentially amyloidogenic proteins of Rhizobium leguminosarum in effective and ineffective root nodules of Pisum sativum L.
Description	We studied potentially amyloidogenic proteins (e.g. protein forming polymers and complexes that are resistant to treatment with ionic detergents) in root nodules formed by two lines of garden pea (P. sativum L.): Sprint-2 (Fix+ phenotype) and Sprint-2Fix- (sym31) (Fix- phenotype) inoculated with the Rhizobium leguminosarum bv. viciae RCAM1026 root nodule bacteria. The Fix+ phenotype is characterized by effective (ability to fix nitrogen) root nodules formation. The Fix- line is a descendant of the Fix+ line and forms ineffective root nodules (unable to fix nitrogen) with undifferentiated bacteroids. We demonstrated the presence of both plant and bacterial proteins in detergent resistant fractions, including previously identified amyloid proteins RopA and RopB of R. leguminosarum and vicilin of P. sativum L.
HostingRepository	PRIDE
AnnounceDate	2022-10-25
AnnouncementXML	Submission_2022-10-25_09:45:54.369.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035724
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	AnastasiiaKosolapova
SpeciesList	scientific name: Rhizobium leguminosarum bv. viciae; NCBI TaxID: 387; scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888;
ModificationList	carbamoylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 02:19:11	ID requested
⏵ 1	2022-10-25 09:45:54	announced

10.6019/PXD035724;

submitter keyword: Pea, PSIA, Pisum sativum,Rhizobium leguminosarum, root nodules, amyloids

AntonNizhnikov
contact affiliation	Laboratory for Proteomics of Supra-Organismal Systems, All-Russia Research Institute for Agricultural Microbiology, St. Petersburg, Russia
contact email	a.nizhnikov@arriam.ru
lab head
AnastasiiaKosolapova
contact affiliation	All-Russian Research Institute for Agricultural Microbiology
contact email	a.kosolapova@arriam.ru
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035724
     1. Label: PRIDE project
     2. Name: Proteomic screening for potentially amyloidogenic proteins of Rhizobium leguminosarum in effective and ineffective root nodules of Pisum sativum L.