PXD035707

PXD035707 is an original dataset announced via ProteomeXchange.

Title	FRIENDLY (FMT) is an RNA binding protein associated with cytosolic ribosomes at the mitochondrial surface
Description	The spatial organization of protein synthesis in the eukaryotic cell is essential for maintaining the integrity of the proteome and the functioning of the cell. Translation on free polysomes or on ribosomes associated with the endoplasmic reticulum has been studied for a long time. More recent data have revealed selective translation of mRNAs in other compartments, in particular at the surface of mitochondria. Although these processes have been described in many organisms, in particular in plants, the mRNA targeting and localized translation mechanisms remain poorly understood. Here, the Arabidopsis thaliana Friendly (FMT) protein is shown to be a cytosolic RNA binding protein that associates with cytosolic ribosomes at the surface of mitochondria. As previously shown (El Zawily et al., 2014), FMT knock-out delays seedling development and causes mitochondrial clustering. The mutation also disrupts the mitochondrial proteome, and the localization of nuclear transcripts encoding mitochondrial proteins at the surface of mitochondria. These data indicate that FMT participates in the localization of mRNAs and their translation at the surface of mitochondria.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:14:35.020.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035707
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Johana Chicher
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 01:38:10	ID requested
1	2023-02-06 04:07:16	announced
⏵ 2	2023-11-14 08:14:37	announced	2023-11-14: Updated project metadata.

Hemono M, Salinas-Gieg, é T, Roignant J, Vingadassalon A, Hammann P, Ubrig E, Ngondo P, Duch, ê, ne AM, FRIENDLY (FMT) is an RNA binding protein associated with cytosolic ribosomes at the mitochondrial surface. Plant J, 112(2):309-321(2022) [pubmed]

10.6019/PXD035707;

submitter keyword: mRNA trafficking, co-translational import, localized translation

Anne-Marie Duchêne
contact affiliation	Institut de biologie moléculaire des plantes, UPR 2357 du CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France
contact email	anne-marie.duchene@ibmp-cnrs.unistra.fr
lab head
Johana Chicher
contact affiliation	Université de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche Scientifique
contact email	j.chicher@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD035707

PRIDE project URI

• PRIDE
  1. PXD035707
     1. Label: PRIDE project
     2. Name: FRIENDLY (FMT) is an RNA binding protein associated with cytosolic ribosomes at the mitochondrial surface